Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | activates | Rhizobium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetosyringone | - |
Rhizobium sp. | |
Ag+ | - |
Rhizobium sp. | |
Ca2+ | 10 mM, slight inhibition | Rhizobium sp. | |
Co2+ | 10 mM, slight inhibition | Rhizobium sp. | |
Cu2+ | - |
Rhizobium sp. | |
dithiothreitol | 50 mM | Rhizobium sp. | |
Fe2+ | - |
Rhizobium sp. | |
Fe3+ | - |
Rhizobium sp. | |
Hg2+ | - |
Rhizobium sp. | |
Li+ | 10 mM, slight inhibition | Rhizobium sp. | |
Mg2+ | 10 mM, slight inhibition | Rhizobium sp. | |
Mn2+ | 10 mM, slight inhibition | Rhizobium sp. | |
SDS | 0.1% | Rhizobium sp. | |
Tris | - |
Rhizobium sp. | |
Urea | 4 M | Rhizobium sp. | |
Zn2+ | 10 mM, slight inhibition | Rhizobium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
4-nitrophenyl alpha-D-glucoside | - |
Rhizobium sp. | |
0.141 | - |
4-methylumbelliferyl-alpha-glucoside | - |
Rhizobium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulates | Rhizobium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
gel filtration | Rhizobium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium sp. | - |
- |
- |
Rhizobium sp. USDA 4280 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhizobium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Rhizobium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.72 | - |
- |
Rhizobium sp. |
Storage Stability | Organism |
---|---|
-20°C, stable for months | Rhizobium sp. |
4°C, stable for several weeks | Rhizobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-glucoside + H2O | - |
Rhizobium sp. | 4-methylumbelliferone + alpha-D-glucose | - |
? | |
4-methylumbelliferyl-alpha-glucoside + H2O | - |
Rhizobium sp. USDA 4280 | 4-methylumbelliferone + alpha-D-glucose | - |
? | |
4-nitrophenyl alpha-D-glucoside | - |
Rhizobium sp. | 4-nitrophenol + alpha-D-glucose | - |
? | |
4-nitrophenyl alpha-D-glucoside | - |
Rhizobium sp. USDA 4280 | 4-nitrophenol + alpha-D-glucose | - |
? | |
maltose + H2O | - |
Rhizobium sp. | alpha-D-glucose + D-glucose | - |
? | |
maltose + H2O | - |
Rhizobium sp. USDA 4280 | alpha-D-glucose + D-glucose | - |
? | |
sucrose + H2O | - |
Rhizobium sp. | D-fructose + alpha-D-glucose | - |
? | |
sucrose + H2O | - |
Rhizobium sp. USDA 4280 | D-fructose + alpha-D-glucose | - |
? | |
trehalose + H2O | - |
Rhizobium sp. | alpha-D-glucose | - |
? | |
trehalose + H2O | - |
Rhizobium sp. USDA 4280 | alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 59000, SDS-PAGE und reducing and under nonreducing conditions | Rhizobium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Rhizobium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | 15°C: about 35% of maximal activity, 50°C: about 25% of maximal activity | Rhizobium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Rhizobium sp. |