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Literature summary for 3.2.1.20 extracted from
- Purification and characterization of a maltase from the extremely thermophilic crenarchaeote Sulfolobus solfataricus (1995), J. Bacteriol., 177, 482-485.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| n-Propanol | maximal stimulation at 7.5% | Saccharolobus solfataricus |  |
General Stability
| General Stability | Organism |
|---|---|
| proteolytic treatment of chemically denatured enzyme generated by guanidine hydrochloride pretreatment results in complete maltase degradation | Saccharolobus solfataricus |
| treatment with trypsin or chymotrypsin at 30°C or proteinase K at 30°C or at 55°C for 20 min in 40 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, pH 7.6, 8 mM magnesium acetate, 0.3 mM EDTA, 2 mM dithiothreitol, 10 mM sodium chloride and 20 mM potassium chloride has no effect | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.91 | - |
maltose | - |
Saccharolobus solfataricus | |
| 3.2 | - |
p-nitrophenyl-alpha-D-glucoside | - |
Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
4 * or 5 * 80000, PAGE after treatment with 6 M guanidine hydrochloride | Saccharolobus solfataricus |
| 80000 | - |
5 * or 4 * 80000, PAGE after treatment with 6 M guanidine hydrochloride | Saccharolobus solfataricus |
| 400000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | 20 min in both 50% n-propanol and 25% ethanol increases maltase activity by over 50% | Saccharolobus solfataricus |
| n-propanol | 20 min in both 50% n-propanol and 25% ethanol increases maltase activity by over 50% | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 120900 | - |
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextrin + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
| isomaltose + H2O | - |
Saccharolobus solfataricus | 2 alpha-D-glucose | - |
? | |
| maltoheptaose + H2O | - |
Saccharolobus solfataricus | maltohexaose + D-glucose | - |
? | |
| maltose + H2O | - |
Saccharolobus solfataricus | alpha-D-glucose + D-glucose | - |
? | |
| maltotetraose + H2O | - |
Saccharolobus solfataricus | alpha-D-glucose | - |
? | |
| maltotriose + H2O | - |
Saccharolobus solfataricus | maltose + D-glucose | - |
? | |
| methyl-alpha-glucoside + H2O | - |
Saccharolobus solfataricus | methanol + alpha-D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + D-glucose | - |
? | |
| starch + H2O | no activity | Saccharolobus solfataricus | alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| pentamer | 5 * or 4 * 80000, PAGE after treatment with 6 M guanidine hydrochloride | Saccharolobus solfataricus |
| tetramer | 4 * or 5 * 80000, PAGE after treatment with 6 M guanidine hydrochloride | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 105 | - |
- |
Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 115 | 65°C: about 30% of maximal activity, 115°C: about 25% of maximal activity | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
half-life: 11 h | Saccharolobus solfataricus |
| 95 | - |
half-life: 3 h | Saccharolobus solfataricus |
| 100 | - |
half-life: 2.75 h | Saccharolobus solfataricus |
| 105 | - |
half-life: 11 h | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Saccharolobus solfataricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 6.5 | about 50% of maximal activity at pH 3.5 and at pH 6.5 | Saccharolobus solfataricus |
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