Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.20 extracted from

  • Piller, K.; Daniel, R.M.; Petach, H.H.
    Properties and stabilization of an extracellular alpha-glucosidase from the extremely thermophilic archaebacteria Thermococcus strain AN1: enzyme activity at 130¦C (1996), Biochim. Biophys. Acta, 1292, 197-205.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
albumin 0.4% w/v, bovine serum albumin, substantially increases activity Thermococcus sp.
dithiothreitol 1%, substantially increases activity Thermococcus sp.

General Stability

General Stability Organism
relatively unaffected by denaturing agents at 75°C Thermococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ slight decrease of activity Thermococcus sp.
Co2+
-
Thermococcus sp.
Hg2+
-
Thermococcus sp.
Mg2+ slight decrease of activity Thermococcus sp.
Ni2+
-
Thermococcus sp.
Zn2+ slight decrease of activity Thermococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
p-nitrophenyl-alpha-D-glucoside free enzyme Thermococcus sp.
0.56
-
p-nitrophenyl-alpha-D-glucoside enzyme immobilized on oxirane acrylic beads Thermococcus sp.
0.71
-
p-nitrophenyl-alpha-D-glucoside cocross-linked enzyme Thermococcus sp.
3
-
p-nitrophenyl-alpha-D-glucoside enzyme immobilized on epoxy carrier Thermococcus sp.
8.2
-
p-nitrophenyl-alpha-D-glucoside enzyme immobilized on uncoated controlled glass beads Thermococcus sp.
8.6
-
p-nitrophenyl-alpha-D-glucoside enzyme immobilized on alkylamino glass beads Thermococcus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermococcus sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
gel filtration Thermococcus sp.
120000
-
x * 120000, SDS-PAGE Thermococcus sp.

Organism

Organism UniProt Comment Textmining
Thermococcus sp.
-
-
-
Thermococcus sp. AN1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80
-
-
Thermococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltose + H2O
-
Thermococcus sp. 2 alpha-D-glucose
-
?
isomaltose + H2O
-
Thermococcus sp. AN1 2 alpha-D-glucose
-
?
maltose + H2O
-
Thermococcus sp. alpha-D-glucose + D-glucose
-
?
maltose + H2O
-
Thermococcus sp. AN1 alpha-D-glucose + D-glucose
-
?
nigerose + H2O
-
Thermococcus sp. 2 alpha-D-glucose
-
?
nigerose + H2O
-
Thermococcus sp. AN1 2 alpha-D-glucose
-
?
p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
Thermococcus sp. 4-nitrophenol + D-glucose
-
?
palatinose + H2O
-
Thermococcus sp. alpha-D-glucose + D-fructose
-
?
palatinose + H2O
-
Thermococcus sp. AN1 alpha-D-glucose + D-fructose
-
?
panose + H2O
-
Thermococcus sp. maltose + alpha-D-glucose
-
?
panose + H2O
-
Thermococcus sp. AN1 maltose + alpha-D-glucose
-
?
turanose + H2O
-
Thermococcus sp. alpha-D-glucose + D-fructose
-
?

Subunits

Subunits Comment Organism
? x * 120000, SDS-PAGE Thermococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
98
-
half-life: 35 min, in presence of 1% w/v dithiothreitol and 1% bovine serum albumin: 215 min Thermococcus sp.
110
-
half-life: 4 min Thermococcus sp.
130
-
in presence of 90% w/v sorbitol, 1% w/v dithiothreitol and 1% w/v bovine serum albumin, half-life: 30-60 min Thermococcus sp.