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Literature summary for 3.2.1.20 extracted from
- Amphiphilic pig intestinal microvillus maltase/glucoamylase. Structure and specificity (1982), Eur. J. Biochem., 126, 559-568.
General Stability
| General Stability | Organism |
|---|---|
| repeated freezing and thawing inactivates | Sus scrofa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.74 | - |
maltose | - |
Sus scrofa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 210000 | - |
enzyme solubilized by papain, gel filtration | Sus scrofa |
| 330000 | - |
enzyme solubilized by Triton X-100, gel filtration | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestine | microvillus | Sus scrofa | - |
| pancreas | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for 1 month | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Sus scrofa | alpha-D-glucose | - |
? | |
| isopanose + H2O | - |
Sus scrofa | alpha-D-glucose | - |
? | |
| kojibiose + H2O | - |
Sus scrofa | alpha-D-glucose + D-glucose | - |
? | |
| maltohexaose + H2O | - |
Sus scrofa | maltopentaose + D-glucose | - |
? | |
| maltose + H2O | - |
Sus scrofa | alpha-D-glucose + D-glucose | - |
? | |
| maltotetraose + H2O | - |
Sus scrofa | alpha-D-glucose | - |
? | |
| maltotriose + H2O | - |
Sus scrofa | maltose + D-glucose | - |
? | |
| nigerose + H2O | - |
Sus scrofa | 2 alpha-D-glucose | - |
? | |
| starch + H2O | - |
Sus scrofa | alpha-D-glucose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Sus scrofa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9 | pH 4.5: about 55% of activity with maltose as substrate, about 30% of activity with starch as substrate, pH 8.0: about 60% of activity with maltose as substrate, about 50% of activity with starch as substrate | Sus scrofa |
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