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Literature summary for 3.2.1.20 extracted from

  • Needleman, R.B.; Federoff, H.J.; Eccleshall, T.R.; Buchferer, B.; Marmur, J.
    Purification and characterization of an alpha-glucosidase from Saccharomyces carlsbergensis (1978), Biochemistry, 17, 4657-4661.
    View publication on PubMed
Search for more literature for 3.2.1.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 p-nitrophenyl-alpha-D-glucoside
-
 Saccharomyces pastorianus
16.6
-
 maltose
-
 Saccharomyces pastorianus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 gel filtration Saccharomyces pastorianus

Organism

Organism UniProt Comment Textmining
Saccharomyces pastorianus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
only a single form of enzyme Saccharomyces pastorianus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
131.3
-
-
 Saccharomyces pastorianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose + H2O
-
 Saccharomyces pastorianus alpha-D-glucose + D-glucose
-
 ?
additional information absolute anomeric specificity for alpha-glycosidic linkages Saccharomyces pastorianus ?
-
 ?
p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
 Saccharomyces pastorianus 4-nitrophenol + D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Saccharomyces pastorianus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
 decay constant: 2.42 per min Saccharomyces pastorianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 6.8 hydrolysis of p-nitrophenyl-alpha-D-glucoside Saccharomyces pastorianus