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Literature summary for 3.2.1.20 extracted from
- Purification and properties of three forms of alpha-glucosidase from germinated green gram (Phaseolus vidissimus Ten.) (1979), Agric. Biol. Chem., 43, 481-489.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | BaCl2 | Vigna radiata |  |
| Co2+ | CoCl2 | Vigna radiata | |
| Cu2+ | Cu(CH3COO)2 | Vigna radiata | |
| EDTA | - |
Vigna radiata | |
| Hg2+ | HgCl2 | Vigna radiata | |
| iodoacetic acid | - |
Vigna radiata | |
| Mn2+ | MnCl2 | Vigna radiata | |
| Ni2+ | NiCl2 | Vigna radiata | |
| Pb2+ | Pb(CH3COO)2 | Vigna radiata | |
| PCMB | - |
Vigna radiata | |
| Tris | - |
Vigna radiata | |
| turanose | - |
Vigna radiata | |
| Zn2+ | ZnCl2 | Vigna radiata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.463 | - |
maltotriose | alpha-glucosidase II-2 | Vigna radiata | |
| 0.485 | - |
maltose | alpha-glucosidase II-1 | Vigna radiata | |
| 0.493 | - |
maltotriose | alpha-glucosidase I | Vigna radiata | |
| 0.584 | - |
maltose | alpha-glucosidase II-2 | Vigna radiata | |
| 0.597 | - |
maltotriose | alpha-glucosidase II-1 | Vigna radiata | |
| 0.714 | - |
amylose | alpha-glucosidase II-1 | Vigna radiata | |
| 0.73 | - |
maltose | alpha-glucosidase I | Vigna radiata | |
| 1 | - |
amylose | alpha-glucosidase I and II-2 | Vigna radiata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, alpha-glucosidase II-2, SDS-PAGE | Vigna radiata |
| 105000 | - |
x * 105000, alpha-glucosidase II-1, SDS-PAGE | Vigna radiata |
| 145000 | - |
x * 145000, alpha-glucosidase I, SDS-PAGE | Vigna radiata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glucosidase I, II-1, and II-2 | Vigna radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose + H2O | - |
Vigna radiata | alpha-D-glucose | - |
? | |
| maltose + H2O | - |
Vigna radiata | alpha-D-glucose + D-glucose | - |
? | |
| maltotriose + H2O | - |
Vigna radiata | maltose + D-glucose | - |
? | |
| phenyl alpha-maltoside + H2O | - |
Vigna radiata | alpha-D-glucose + phenyl alpha-D-glucoside | - |
? | |
| starch + H2O | soluble | Vigna radiata | alpha-D-glucose | - |
? | |
| sucrose + H2O | no activity | Vigna radiata | D-fructose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 105000, alpha-glucosidase II-1, SDS-PAGE | Vigna radiata |
| ? | x * 145000, alpha-glucosidase I, SDS-PAGE | Vigna radiata |
| ? | x * 65000, alpha-glucosidase II-2, SDS-PAGE | Vigna radiata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
alpha-glucosidase I | Vigna radiata |
| 65 | - |
alpha-glucosidase II-1 and II-2 | Vigna radiata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
15 min, stable up to | Vigna radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Vigna radiata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 6.5 | 30°C, 20 h, stable | Vigna radiata |
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