Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
E380Q | structural modeling of BAM-9. BAM-4 and BAM-9 are also substituted at position 342 on the inner loop | Arabidopsis thaliana |
E380R | structural modeling of the BAM-4 active site predicts an inactive protein. BAM-4 and BAM-9 are also substituted at position 342 on the inner loop | Arabidopsis thaliana |
additional information | bam1, T-DNA insertion mutation, has no elevated starch levels and no lower nighttime maltose levels than the wild type. Mutant bam2, T-DNA insertion mutation. Mutant bam3, formation of a mutant line via the Arabidopsis TILLING Program, has elevated starch levels and lower nighttime maltose levels than the wild type. Mutant bam4, T-DNA insertion mutation, has elevated starch levels. Double mutant bam1 bam3, more severe phenotype than bam3. Total beta-amylase activity is reduced in leaves of bam1 and bam3 mutants but not in bam2 and bam4 mutants | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | BAM-1, BAM-2, BAM-3, BAM-4 | Arabidopsis thaliana | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54600 | - |
BAM-2 | Arabidopsis thaliana |
55500 | - |
BAM-3 | Arabidopsis thaliana |
55500 | - |
calculated, BAM-2 | Arabidopsis thaliana |
55800 | - |
calculated, BAM-3 | Arabidopsis thaliana |
59500 | - |
calculated, BAM-1 | Arabidopsis thaliana |
67500 | - |
BAM-1 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | Arabidopsis thaliana | - |
beta-maltose + ? | - |
? | |
maltotetraose + H2O | Arabidopsis thaliana | - |
? | - |
? | |
starch + H2O | Arabidopsis thaliana | - |
beta-maltose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia | - |
Purification (Comment) | Organism |
---|---|
affinity chromatography, to near homogeneity using Glutathione-Sepharose 4B | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
BAM-2 specific activity is 25fold lower than that of BAM-3 and 50fold lower than that of BAM-1. No activity is detectable for recombinant BAM-4 protein | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Arabidopsis thaliana | beta-maltose + ? | - |
? | |
maltotetraose + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
starch + H2O | - |
Arabidopsis thaliana | beta-maltose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BAM-1 | chloroplastic | Arabidopsis thaliana |
BAM-2 | chloroplastic | Arabidopsis thaliana |
BAM-3 | chloroplastic | Arabidopsis thaliana |
BAM-5 | - |
Arabidopsis thaliana |
BAM-6 | - |
Arabidopsis thaliana |
BAM-7 | - |
Arabidopsis thaliana |
BAM-8 | - |
Arabidopsis thaliana |
BAM-9 | - |
Arabidopsis thaliana |
beta-amylase | - |
Arabidopsis thaliana |
More | BAM-4 is chloroplastic, expressed in Escherichia coli with no beta-amylase activity, probably lost its catalytic capacity during evolution | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Arabidopsis thaliana |