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Literature summary for 3.2.1.199 extracted from
- YihQ is a sulfoquinovosidase that cleaves sulfoquinovosyl diacylglyceride sulfolipids (2016), Nat. Chem. Biol., 12, 215-217.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structure reveals an (alphabeta)8 barrel appended with a small beta-sheet domain. Residue D405 fulfills the role of catalytic nucleophile and D472 acts as a general acid-base | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D405N | inactive | Escherichia coli |
| D472A | inactive | Escherichia coli |
| D472N | inactive | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-fluoro-beta-L-idopyranosyl fluoride | mechanism-based inactivator, soaking of crystals yields a covalent glycosyl-enzyme complex in a 1S3 pyranose conformation | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
4-nitrophenyl alpha-sulfoquinovoside | pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a sulfoquinovosyl diacylglyceride + H2O | Escherichia coli | - |
6-sulfo-D-quinovose + a diacylglycerol | complete conversion to sulfoquinose | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P32138 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-sulfoquinovosylglycerol + H2O | - |
Escherichia coli | 6-sulfo-D-quinovose + glycerol | - |
? | |
| 4-nitrophenyl alpha-sulfoquinovoside + H2O | - |
Escherichia coli | 6-sulfo-D-quinovose + 4-nitrophenol | - |
? | |
| a sulfoquinovosyl diacylglyceride + H2O | - |
Escherichia coli | 6-sulfo-D-quinovose + a diacylglycerol | complete conversion to sulfoquinose | ? | |
| additional information | no substrate: 4-nitrophenyl alpha-D-glucopyranoside | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| yihQ | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.3 | - |
4-nitrophenyl alpha-sulfoquinovoside | pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 64 | - |
4-nitrophenyl alpha-sulfoquinovoside | pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
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Release 2023.1 (January 2023)
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