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Literature summary for 3.2.1.197 extracted from

  • Cuskin, F.; Basle, A.; Ladeveze, S.; Day, A.; Gilbert, H.; Davies, G.; Potocki-Veronese, G.; Lowe, E.
    The GH130 family of mannoside phosphorylases contains glycoside hydrolases that target beta-1,2-mannosidic linkages in Candida mannan (2015), J. Biol. Chem., 290, 25023-25033.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.197

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with mannose bound in the -1 and +1 subsites. A pair of glutamate residues, Glu227 and Glu268, hydrogen bond to O1 of alpha-mannose, and either of these residues may function as the catalytic base. Conserved lysine residue, Lys199 is a key specificity determinant for beta-1,2-mannosidic linkages Bacteroides thetaiotaomicron

Protein Variants

Protein Variants Comment Organism
D142N no activity Bacteroides thetaiotaomicron
D363A no activity Bacteroides thetaiotaomicron
E141Q no activity Bacteroides thetaiotaomicron
E227Q 32% of wild-type activity Bacteroides thetaiotaomicron
E227Q/E268Q no activity Bacteroides thetaiotaomicron
E268A 5% of wild-type activity Bacteroides thetaiotaomicron
E268Q 4% of wild-type activity Bacteroides thetaiotaomicron
F344A 31% of wild-type activity Bacteroides thetaiotaomicron
K199A no activity Bacteroides thetaiotaomicron
N74A 15% of wild-type activity Bacteroides thetaiotaomicron
R89A 25% of wild-type activity Bacteroides thetaiotaomicron
Y302A 6% of wild-type activity Bacteroides thetaiotaomicron

Organism

Organism UniProt Comment Textmining
Bacteroides ovatus A7M0J4
-
-
Bacteroides ovatus DSM 1896 A7M0J4
-
-
Bacteroides thetaiotaomicron Q8A186
-
-
Bacteroides thetaiotaomicron DSM 2079 Q8A186
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
 ?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
 ?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration ?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration ?
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron beta-D-mannopyranose + alpha-D-mannopyranose
-
 ?
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 beta-D-mannopyranose + alpha-D-mannopyranose
-
 ?
Candida mannan + H2O
-
 Bacteroides thetaiotaomicron ? + alpha-D-mannopyranose
-
 ?
Candida mannan + H2O
-
 Bacteroides ovatus ? + alpha-D-mannopyranose
-
 ?
Candida mannan + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 ? + alpha-D-mannopyranose
-
 ?
Candida mannan + H2O
-
 Bacteroides ovatus DSM 1896 ? + alpha-D-mannopyranose
-
 ?
additional information enzyme is a beta-mannosidase that hydrolyzes beta-1,2-mannosidic linkages through an inverting mechanism Bacteroides thetaiotaomicron ?
-
 ?
additional information enzyme is a beta-mannosidase that hydrolyzes beta-1,2-mannosidic linkages through an inverting mechanism Bacteroides thetaiotaomicron DSM 2079 ?
-
 ?

Synonyms

Synonyms Comment Organism
BACOVA_03624
-
 Bacteroides ovatus
BT_3780
-
 Bacteroides thetaiotaomicron

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
 beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 7.5., 37°C Bacteroides thetaiotaomicron
0.06
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 7.5., 37°C Bacteroides thetaiotaomicron
0.085
-
 beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose pH 7.5., 37°C Bacteroides thetaiotaomicron
0.1
-
 Candida mannan pH 7.5., 37°C Bacteroides ovatus
0.115
-
 Candida mannan pH 7.5., 37°C Bacteroides thetaiotaomicron