Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
4 * 110000, His6-tagged enzyme, SDS-PAGE | Corynebacterium glutamicum |
417000 | - |
native enzyme, gel filtration | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphorylase-limit dextrin | Corynebacterium glutamicum | - |
maltodextrin + ? | - |
? | |
phosphorylase-limit dextrin | Corynebacterium glutamicum ATCC 13032 | - |
maltodextrin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Corynebacterium glutamicum ATCC 13032 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Corynebacterium glutamicum | maltose | - |
? | |
amylopectin + H2O | - |
Corynebacterium glutamicum ATCC 13032 | maltose | - |
? | |
glycogen + H2O | - |
Corynebacterium glutamicum | maltose + ? | - |
? | |
glycogen + H2O | - |
Corynebacterium glutamicum ATCC 13032 | maltose + ? | - |
? | |
phosphorylase-limit dextrin | - |
Corynebacterium glutamicum | maltodextrin + ? | - |
? | |
phosphorylase-limit dextrin | - |
Corynebacterium glutamicum ATCC 13032 | maltodextrin + ? | - |
? | |
soluble starch + H2O | - |
Corynebacterium glutamicum | maltose + ? | - |
? | |
soluble starch + H2O | - |
Corynebacterium glutamicum ATCC 13032 | maltose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 110000, His6-tagged enzyme, SDS-PAGE | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
GlgX | - |
Corynebacterium glutamicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
the His6-tagged enzyme is inactivated by incubation at 95°C for 10 min | Corynebacterium glutamicum |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivation of glgX leads to slower growth and to a higher intracellular glycogen pool throughout growth, when compared to those in the parental strain | Corynebacterium glutamicum |
physiological function | the enzyme is required for glycogen degradation and for fast adaptation to hyperosmotic stress | Corynebacterium glutamicum |