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Literature summary for 3.2.1.196 extracted from

  • Dauvillee, D.; Kinderf, I.S.; Li, Z.; Kosar-Hashemi, B.; Samuel, M.S.; Rampling, L.; Ball, S.; Morell, M.K.
    Role of the Escherichia coli glgX gene in glycogen metabolism (2005), J. Bacteriol., 187, 1465-1473.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.196

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21AI cells Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 x * 76000, His6-tagged enzyme, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli BW25113
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the isoamylase-type debranching enzyme shows high specificity for hydrolysis of chains consisting of three or four glucose residues Escherichia coli ?
-
 ?
additional information the isoamylase-type debranching enzyme shows high specificity for hydrolysis of chains consisting of three or four glucose residues Escherichia coli BW25113 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 76000, His6-tagged enzyme, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
GlgX
-
 Escherichia coli
isoamylase-type debranching enzyme
-
 Escherichia coli

General Information

General Information Comment Organism
metabolism the enzyme is predominantly involved in glycogen catabolism by selectively debranching the polysaccharide outer chains that were previously recessed by glycogen phosphorylase Escherichia coli