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Literature summary for 3.2.1.194 extracted from

  • Wang, D.M.; Yu, H.S.; Song, J.G.; Xu, Y.F.; Liu, C.Y.; Jin, F.X.
    A novel ginsenosidase from an Aspergillus strain hydrolyzing 6-O-multi-glycosides of protopanaxatriol-type ginsenosides, named ginsenosidase type IV (2011), J. Microbiol. Biotechnol., 21, 1057-1063.
    View publication on PubMed
Search for more literature for 3.2.1.194

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Aspergillus sp.
Fe2+
-
 Aspergillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 50-100 mM, slightly improves activity Aspergillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Aspergillus sp.

Organism

Organism UniProt Comment Textmining
Aspergillus sp.
-
-
-
Aspergillus sp. 39g
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.68
-
 pH 6.0, 40°C Aspergillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ginsenoside R1 + H2O the enzyme hydrolyzes the 6-O-D-(1->2)-xyloside Aspergillus sp. ginsenoside Rg1 + D-xylose
-
 ?
ginsenoside R1 + H2O the enzyme hydrolyzes the 6-O-D-(1->2)-xyloside Aspergillus sp. 39g ginsenoside Rg1 + D-xylose
-
 ?
ginsenoside Re + H2O the enzyme hydrolyzes the 6-O-alpha-L-(1->2)-rhamnoside Aspergillus sp. ginsenoside Rg1 + L-rhamnose
-
 ?
ginsenoside Re + H2O the enzyme hydrolyzes the 6-O-alpha-L-(1->2)-rhamnoside Aspergillus sp. 39g ginsenoside Rg1 + L-rhamnose
-
 ?
ginsenoside Rf + H2O the enzyme hydrolyzes 6-O-beta-D-(1->2)-glucoside Aspergillus sp. ginsenoside Rh1 + D-glucose
-
 ?
ginsenoside Rf + H2O the enzyme hydrolyzes 6-O-beta-D-(1->2)-glucoside Aspergillus sp. 39g ginsenoside Rh1 + D-glucose
-
 ?
ginsenoside Rg1 + H2O the enzyme weakly hydrolyzes 6-O-beta-D-glucoside Aspergillus sp. ginsenoside F1 + D-glucose
-
 ?
ginsenoside Rg1 + H2O the enzyme weakly hydrolyzes 6-O-beta-D-glucoside Aspergillus sp. 39g ginsenoside F1 + D-glucose
-
 ?
ginsenoside Rg2 + H2O the enzyme hydrolyzes 6-O-alpha-L-(1->2)-rhamnoside Aspergillus sp. ginsenoside Rh1 + L-rhamnose
-
 ?
ginsenoside Rh1 + H2O the enzyme weakly hydrolyzes 6-O-beta-D-glucoside Aspergillus sp. protopanaxatriol + D-glucose
-
 ?
additional information ginsenosidase type IV can not hydrolyze the 3-O- or 20-O-glycosides of protopanaxadiol type ginsenosides (PPD), such as Rb1, Rb2, Rb3, Rc, and Rd Aspergillus sp. ?
-
 ?
additional information ginsenosidase type IV can not hydrolyze the 3-O- or 20-O-glycosides of protopanaxadiol type ginsenosides (PPD), such as Rb1, Rb2, Rb3, Rc, and Rd Aspergillus sp. 39g ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Aspergillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Aspergillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 70% of maximal activity, 60°C: about 80% of maximal activity Aspergillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 8 pH 3.0: about 70% of maximal activity, pH 8.0: about 80% of maximal activity Aspergillus sp.