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Literature summary for 3.2.1.190 extracted from

  • Qian, S.; Yu, H.; Zhang, C.; Lu, M.; Wang, H.; Jin, F.
    Purification and characterization of dioscin-alpha-L-rhamnosidase from pig liver (2005), Chem. Pharm. Bull., 53, 911-914.
    View publication on PubMed
Search for more literature for 3.2.1.190

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 10 mM, 94% inhibition Sus scrofa
Fe3+ 10 mM, 88% inhibition Sus scrofa
Mg2+ inhibits at 10-100 mM, slightly activates at 200 mM Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10-200 mM, 1.13-1.18fold activation Sus scrofa
Mg2+ inhibits at 10-100 mM, slightly activates (109% of the control activity) at 200 mM Sus scrofa
Zn2+ 10-200 mM, 1.08-1.1fold activation Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 47000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O Sus scrofa the enzyme is involved in hydrolysis of the steroid saponin dioscin 2 L-rhamnopyranose + diosgenin 3-O-beta-D-glucopyranoside
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
88
-
 pH 7.2, 37°C Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O the enzyme is involved in hydrolysis of the steroid saponin dioscin Sus scrofa 2 L-rhamnopyranose + diosgenin 3-O-beta-D-glucopyranoside
-
 ?
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O
-
 Sus scrofa 2 L-rhamnopyranose + diosgenin-3-O-beta-D-glucopyranoside
-
 ?
additional information no hydrolysis of ginsenoside Re or rutin Sus scrofa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
dioscin alpha-L-rhamnosidase
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
 Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
32 57 32°C: about 85% of maximal activity, 57°C: about 35% of maximal activity Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
32 52 stable Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
4 9 pH 4.0: about 50% of maximal activity, pH 9.0: about 60% of maximal activity Sus scrofa