Cloned (Comment) | Organism |
---|---|
gene bgl3, DNA and amino acid sequence determiantion and analysis, sequence comparisons, quantitative real-time PCR expression analysis, recombinant expression of His-tagged enzyme in Pichia pastoris with post-translational glycosylation | Solanum torvum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
torvoside A | native enzyme, pH 5.0, 37°C | Solanum torvum | |
0.069 | - |
torvoside A | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum | |
0.66 | - |
4-methylumbelliferyl beta-D-glucoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum | |
0.78 | - |
4-methylumbelliferyl beta-D-glucoside | native enzyme, pH 5.0, 37°C | Solanum torvum | |
1.01 | - |
4-nitrophenyl beta-D-glucoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum | |
1.03 | - |
4-nitrophenyl beta-D-glucoside | native enzyme, pH 5.0, 37°C | Solanum torvum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
native enzyme, gel filtration | Solanum torvum |
169500 | - |
glycosylated recombinant His-tagged enzyme, gel filtration | Solanum torvum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Solanum torvum | the beta-glucosidase (torvosidase) purified from Solanum torvum leaves specifically catalyzes the hydrolysis of two furostanol 26-O-beta-glucosides, torvosides A and H | ? | - |
? | |
torvoside A + H2O | Solanum torvum | - |
26-deglucotorvoside A + D-glucose | - |
? | |
torvoside H + H2O | Solanum torvum | - |
26-deglucotorvoside H + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum torvum | P0DKH4 | i.e. Solanum ficifolium | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the native enzyme is glycosylated, while the recombinant enzyme is hyperglycosylated, deglycosylation by endoglycosidase H (EndoH) shifting the molecular weight from 140 to 70 kDa | Solanum torvum |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 323fold from Pichia pastoris by nickel affinity chromatography | Solanum torvum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | high expression level | Solanum torvum | - |
additional information | quantitative real-time PCR expression analysis | Solanum torvum | - |
seed | germinating, high expression level | Solanum torvum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
purified recombinant His-tagged enzyme, substrate torvoside A, pH 5.0, 37°C | Solanum torvum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-glucoside + H2O | - |
Solanum torvum | 4-methylumbelliferol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Solanum torvum | 4-nitrophenol + beta-D-glucose | - |
? | |
decyl-beta-D-glucoside + H2O | 83% activity compared to torvoside A | Solanum torvum | decanol + D-glucose | - |
? | |
hexyl-beta-D-glucoside + H2O | 43% activity compared to torvoside A | Solanum torvum | hexanol + D-glucose | - |
? | |
additional information | the beta-glucosidase (torvosidase) purified from Solanum torvum leaves specifically catalyzes the hydrolysis of two furostanol 26-O-beta-glucosides, torvosides A and H | Solanum torvum | ? | - |
? | |
additional information | the beta-glucosidase (torvosidase) purified from Solanum torvum leaves specifically catalyzes the hydrolysis of two furostanol 26-O-beta-glucosides, torvosides A and H. No activity with (1,2)-beta-linked glucobiose and (1,3)-beta-linked glucobiose, and with beta-linked oligosaccharides, namely cellobiose, cellotriose, cellotetraose, laminaribiose, laminaritriose, laminaritetraose, and gentiobiose | Solanum torvum | ? | - |
? | |
octyl-beta-D-glucoside + H2O | 69% activity compared to torvoside A | Solanum torvum | octanol + D-glucose | - |
? | |
phenyl-beta-D-glucoside + H2O | 36% activity compared to torvoside A | Solanum torvum | phenol + D-glucose | - |
? | |
torvoside A + H2O | - |
Solanum torvum | 26-deglucotorvoside A + D-glucose | - |
? | |
torvoside A + H2O | best substrate for native and recombinant enzymes | Solanum torvum | 26-deglucotorvoside A + D-glucose | - |
? | |
torvoside H + H2O | - |
Solanum torvum | 26-deglucotorvoside H + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66573, sequence calculation, 1 * 80700, native enzyme, SDS-PAGE, 1 * 140000, glycosylated recombinant His-tagged enzyme, SDS-PAGE, 1 * 70000, deglycosylated recombinant His-tagged enzyme, SDS-PAGE | Solanum torvum |
Synonyms | Comment | Organism |
---|---|---|
S. torvum beta-glucosidase | - |
Solanum torvum |
SBgl3 | - |
Solanum torvum |
Solanum torvum GH3 beta-glucosidase | - |
Solanum torvum |
torvosidase | - |
Solanum torvum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Solanum torvum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
4-methylumbelliferyl beta-D-glucoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum | |
4.9 | - |
4-nitrophenyl beta-D-glucoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum | |
7.3 | - |
torvoside A | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum | |
8.3 | - |
4-methylumbelliferyl beta-D-glucoside | native enzyme, pH 5.0, 37°C | Solanum torvum | |
8.6 | - |
torvoside A | native enzyme, pH 5.0, 37°C | Solanum torvum | |
9.1 | - |
4-nitrophenyl beta-D-glucoside | native enzyme, pH 5.0, 37°C | Solanum torvum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | recombinant His-tagged enzyme | Solanum torvum |
5 | - |
native enzyme | Solanum torvum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Solanum torvum | sequence calculation | - |
8.07 |
General Information | Comment | Organism |
---|---|---|
evolution | torvosidase is classified as a glycosyl hydrolase family GH3 beta-glucosidase, but cannot hydrolyze beta-oligoglucosides, the natural substratesof GH3 enzymes. Its substrates, furostanol glycoside 26-O-beta-glucosides, are reported as natural substrates of some plant GH1 enzymes | Solanum torvum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.85 | - |
4-nitrophenyl beta-D-glucoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum | |
7.12 | - |
4-methylumbelliferyl beta-D-glucoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum | |
8.8 | - |
4-nitrophenyl beta-D-glucoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum | |
10.6 | - |
4-methylumbelliferyl beta-D-glucoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum | |
105.8 | - |
torvoside A | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum | |
136.5 | - |
torvoside A | recombinant His-tagged enzyme, pH 5.0, 37°C | Solanum torvum |