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Literature summary for 3.2.1.186 extracted from

  • Arthan, D.; Kittakoop, P.; Esen, A.; Svasti, J.
    Furostanol glycoside 26-O-beta-glucosidase from the leaves of Solanum torvum (2006), Phytochemistry, 67, 27-33.
    View publication on PubMed
Search for more literature for 3.2.1.186

Inhibitors

Inhibitors Comment Organism Structure
D-glucono-1,5-lactone 1 mM, 44% inhibition. 10 mM, 91% inhibition Solanum torvum
Hg2+ 1 mM, 88% loss of activity Solanum torvum
Mg2+ 1 mM, 10% inhibition Solanum torvum
Zn2+ 1 mM, 12% inhibition Solanum torvum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
 torvoside A pH 5.0, 37°C Solanum torvum
0.068
-
 torvoside H pH 5.0, 37°C Solanum torvum
0.78
-
 4-methylumbelliferyl beta-D-glucoside pH 5.0, 37°C Solanum torvum
1.03
-
 4-nitrophenyl beta-D-glucoside pH 5.0, 37°C Solanum torvum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80700
-
 1 * 80700, SDS-PAGE Solanum torvum
87000
-
 gel filtration Solanum torvum

Organism

Organism UniProt Comment Textmining
Solanum torvum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Solanum torvum

Purification (Commentary)

Purification (Comment) Organism
-
 Solanum torvum

Source Tissue

Source Tissue Comment Organism Textmining
leaf young leaves Solanum torvum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-glucoside + H2O
-
 Solanum torvum 4-methylumbelliferol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Solanum torvum 4-nitrophenol + D-glucose
-
 ?
dalcochinin 8'-beta-D-glucoside + H2O the natural substrate of Thai rosewood beta-glucosidase. 24% of the activity with torvoside A Solanum torvum ?
-
 ?
additional information no activity with 4-nitrophenyl beta-D-fucoside, 4-nitrophenyl beta-L-fucoside, 4-nitrophenyl beta-D-xyloside, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-N-acetylglucosaminide, 4-nitrophenyl beta-D-mannoside, amygdalin, laminarin, prunasin, glucosinolate (sinigrin), aromatic glucosides (arbutin, salicin), indoxyl-beta-D-glucoside, methyl-beta-D-glucoside, cellobiose, cellotriose, cellotetraose, laminaribiose, laminaritriose, laminaritetraose and gentiobiose Solanum torvum ?
-
 ?
torvoside A
-
 Solanum torvum 26-deglucotorvoside A + D-glucose
-
 ?
torvoside H
-
 Solanum torvum 26-deglucotorvoside H + D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 80700, SDS-PAGE Solanum torvum

Synonyms

Synonyms Comment Organism
torvosidase
-
 Solanum torvum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Solanum torvum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.7
-
 torvoside H pH 5.0, 37°C Solanum torvum
8.3
-
 4-methylumbelliferyl beta-D-glucoside pH 5.0, 37°C Solanum torvum
8.6
-
 torvoside A pH 5.0, 37°C Solanum torvum
9.1
-
 4-nitrophenyl beta-D-glucoside pH 5.0, 37°C Solanum torvum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 reaction with p-nitrophenyl beta-D-glucoside or torvoside H Solanum torvum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum torvum isoelectric focusing
-
 8.8

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.8
-
 4-nitrophenyl beta-D-glucoside pH 5.0, 37°C Solanum torvum
10.7
-
 4-methylumbelliferyl beta-D-glucoside pH 5.0, 37°C Solanum torvum
113
-
 torvoside H pH 5.0, 37°C Solanum torvum
136.5
-
 torvoside A pH 5.0, 37°C Solanum torvum