Literature summary for 3.2.1.184 extracted from

Glaze, P.A.; Watson, D.C.; Young, N.M.; Tanner, M.E.
Biosynthesis of CMP-N,N-diacetyllegionaminic acid from UDP-N,N-diacetylbacillosamine in Legionella pneumophila (2008), Biochemistry, 47, 3272-3282.

Search for more literature for 3.2.1.184

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in Escherichia coli as a His-tagged fusion protein	Legionella pneumophila

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0365	-	UDP-N,N'-diacetylbacillosamine	pH 7.5, 37°C	Legionella pneumophila

Organism

Organism	UniProt	Comment	Textmining
Legionella pneumophila	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Legionella pneumophila

Reaction

Reaction	Comment	Organism	Reaction ID
UDP-N,N'-diacetylbacillosamine + H2O = UDP + 2,4-diacetamido-2,4,6-trideoxy-D-mannopyranose	the reaction proceeds with an inversion of stereochemistry at C-2 and retention of stereochemistry at C-1. Evidence of the cleavage of the C-1-O bond and the C-2-H bond supports an elimination/hydration mechanism similar to that of NeuC in sialic acid bisoynthesis	Legionella pneumophila	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	when UDP-GlcNAc is incubated with the enzyme, no product formation is observed	Legionella pneumophila	?	-	?
UDP-N,N'-diacetylbacillosamine + H2O	-	Legionella pneumophila	2,4-diacetamido-2,4,6-trideoxymannose + UDP	-	?

Synonyms

Synonyms	Comment	Organism
neuC	-	Legionella pneumophila
UDP-N-acetylglucosamine 2-epimerase	-	Legionella pneumophila

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Legionella pneumophila

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
59.1	-	UDP-N,N'-diacetylbacillosamine	pH 7.5, 37°C	Legionella pneumophila

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Legionella pneumophila

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Release 2023.1 (January 2023)