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Literature summary for 3.2.1.182 extracted from

  • Dick, R.; Rattei, T.; Haslbeck, M.; Schwab, W.; Gierl, A.; Frey, M.
    Comparative analysis of benzoxazinoid biosynthesis in monocots and dicots: independent recruitment of stabilization and activation functions (2012), Plant Cell, 24, 915-928.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.182

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis, heterologus expression Consolida orientalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6
-
 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
4.8
-
 Dhurrin recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
28.4
-
 (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O Consolida orientalis
-
 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Consolida orientalis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Consolida orientalis
-
flower bud
-
 Consolida orientalis
-
leaf of all ages Consolida orientalis
-
additional information no expression in roots Consolida orientalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
-
 Consolida orientalis (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
 ?
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O preferred substrate Consolida orientalis (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
 ?
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O low activity Consolida orientalis (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
 ?
dhurrin + H2O
-
 Consolida orientalis ?
-
 ?

Synonyms

Synonyms Comment Organism
Co-Glu
-
 Consolida orientalis
GDIBOA-glucosidase
-
 Consolida orientalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
 (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
32
-
 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
52
-
 Dhurrin recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside hydrolase family 1, GH1, phylogenetic analysis, overview Consolida orientalis
metabolism the enzyme is involved in the benzoxazinoid pathway, overview Consolida orientalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.15
-
 (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
7
-
 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis
10.8
-
 Dhurrin recombinant enzyme, pH and temperature not specified in the publication Consolida orientalis