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Literature summary for 3.2.1.180 extracted from

  • Nakamichi, Y.; Maruyama, Y.; Mikami, B.; Hashimoto, W.; Murata, K.
    Structural determinants in streptococcal unsaturated glucuronyl hydrolase for recognition of glycosaminoglycan sulfate groups (2011), J. Biol. Chem., 286, 6262-6271.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.180

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus agalactiae

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor-diffusion method Streptococcus agalactiae

Protein Variants

Protein Variants Comment Organism
K370I kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 28% of the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is 5fold higher than wild-type value Streptococcus agalactiae
S365H kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 1.6% of the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to wild-type value Streptococcus agalactiae
S368G kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 130% of the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is 3.6fold higher than the wild-type value Streptococcus agalactiae
T235A kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 2% of the wild-type value Streptococcus agalactiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
0.191
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae
0.371
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae
0.763
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae
1.27
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
1.4
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae
1.47
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae
1.75
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme T235A Streptococcus agalactiae
2.36
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae

Organism

Organism UniProt Comment Textmining
Streptococcus agalactiae Q8E372
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus agalactiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-4-deoxy-DELTA4,5-IdoA-(1->4)-beta-D-GlcNAc + H2O
-
 Streptococcus agalactiae alpha-D-4-deoxy-DELTA4,5-IdoA + beta-D-N-acetylglucosamine
-
 ?
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O kcat/Km is 2% of the kcat/Km-value for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S Streptococcus agalactiae 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine
-
 ?
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O
-
 Streptococcus agalactiae 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine-6-O-sulfate
-
 ?
additional information the enzyme acts on unsaturated alpha-iduronic acid as well as unsaturated beta-glucuronic acis residues in substrates, and cleaves both glycoside bonds (1->3) and (1->4) Streptococcus agalactiae ?
-
 ?

Synonyms

Synonyms Comment Organism
UGL ambiguous Streptococcus agalactiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptococcus agalactiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.69
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae
2.69
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
3.85
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae
3.96
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme T235A Streptococcus agalactiae
3.97
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae
10.2
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
10.8
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae
24.8
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae
41.6
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Streptococcus agalactiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.63
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae
2.12
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
2.21
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S365H Streptococcus agalactiae
2.26
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme T235A Streptococcus agalactiae
7.71
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae
10.7
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae
28.2
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme K370I Streptococcus agalactiae
102
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, wild-type enzyme Streptococcus agalactiae
130
-
 beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S pH 7.5, 30°C, mutant enzyme S368G Streptococcus agalactiae