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Literature summary for 3.2.1.18 extracted from

  • Yang, Y.; Chang, S.; Gong, X.; Wu, J.; Liu, B.
    Expression, purification and characterization of low-glycosylation influenza neuraminidase in alpha-1,6-mannosyltransferase defective Pichia pastoris (2011), Mol. Biol. Rep., 39, 857-864.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged viral neuraminidase in wild-type Pichia pastoris strain GS115, an alpha-1,6-mannosyltransferase (och1)-defective Pichia pastoris mutant strain, and in Escherichia coli, resulting in a hyper-glycosylated, a low-glycosylated, and a non-glycosylated enzyme, respectively influenza A virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 59000-97000, hyperglycosylated recombinant enzyme, SDS-PAGE, x * 66000, low-glycosylated recombinant enzyme, SDS-PAGE, x * 45000, non-glycosylated recombinant enzyme, SDS-PAGE influenza A virus
66000
-
x * 59000-97000, hyperglycosylated recombinant enzyme, SDS-PAGE, x * 66000, low-glycosylated recombinant enzyme, SDS-PAGE, x * 45000, non-glycosylated recombinant enzyme, SDS-PAGE influenza A virus

Organism

Organism UniProt Comment Textmining
influenza A virus Q7TG96
-
-
influenza A virus newCaledonia/20/99(H1N1) Q7TG96
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged viral neuraminidase by nickel affinity chromatography, desalting gel filtration, and cation exchange chromatography influenza A virus

Subunits

Subunits Comment Organism
? x * 59000-97000, hyperglycosylated recombinant enzyme, SDS-PAGE, x * 66000, low-glycosylated recombinant enzyme, SDS-PAGE, x * 45000, non-glycosylated recombinant enzyme, SDS-PAGE influenza A virus

Synonyms

Synonyms Comment Organism
neuraminidase
-
influenza A virus

General Information

General Information Comment Organism
additional information low-glycosylation in och1-defective Pichia pastoris enhances the immunogenicity of recombinant neuraminidase and elicits similar antibody titers with less antigen when compared with hyper- and non-glycosylated neuraminidases after vaccination of mice influenza A virus