Activating Compound | Comment | Organism | Structure |
---|---|---|---|
galactose | activates | Entamoeba histolytica | |
lactose | activates | Entamoeba histolytica | |
additional information | not affected by glucose, sucrose, and mannose, the motility of the organism is increased by about 6fold in vivo in presence of fetuin, alpha(2-3)-sialyllactose, and 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-didehydroneuraminic acid | - |
Entamoeba histolytica | |
4-nitrophenyl oxamic acid | - |
Entamoeba histolytica | |
Cu2+ | - |
Entamoeba histolytica | |
Fe2+ | - |
Entamoeba histolytica | |
additional information | the motility of the organism is decreased in vivo in presence of 2,3-didehydroneuraminic acid and sialic acid containing compounds | Entamoeba histolytica | |
Zn2+ | - |
Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | pH 5.5, 37°C | Entamoeba histolytica | |
0.144 | - |
alpha(2-3)-sialyllactose | pH 5.5, 37°C | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Entamoeba histolytica | |
Mg2+ | activates | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
677fold, to homogeneity by chromatography on hydroxyapatite, fetuin-agarose, and Mono Q | Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.05 | - |
purified enzyme | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Entamoeba histolytica | 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid | - |
? | |
alpha(2-3)-sialyllactose + H2O | - |
Entamoeba histolytica | sialic acid + lactose | - |
? | |
alpha(2-6)-sialyllactose + H2O | low activity | Entamoeba histolytica | sialic acid + lactose | - |
? | |
fetuin + H2O | - |
Entamoeba histolytica | sialic acid + ? | - |
? | |
additional information | colominic acid, and gangliosides GM1 and GD1 are poor substrates | Entamoeba histolytica | ? | - |
? | |
mucin + H2O | - |
Entamoeba histolytica | sialic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
sialidase | - |
Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Entamoeba histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
2,3-didehydroneuraminic acid | pH 5.5, 37°C | Entamoeba histolytica | |
0.185 | - |
4-nitrophenyl oxamic acid | pH 5.5, 37°C | Entamoeba histolytica |