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Literature summary for 3.2.1.18 extracted from
- Sialidase, receptor-binding and fusion-promotion activities of Newcastle disease virus haemagglutinin-neuraminidase glycoprotein: a mutational and kinetic study (2004), J. Gen. Virol., 85, 1981-1988.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transient expression of wild-type and mutant enzymes in HeLa cells, cell surface orientation | Avian orthoavulavirus 1 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E258D | site-directed mutagenesis, mutation of a catalytic residue, highly reduced sialidase activity, slightly reduced HA activity, mutant shows reduced fusion promotion activity | Avian orthoavulavirus 1 |
| R498K | site-directed mutagenesis, mutation of a catalytic residue, reduced sialidase activity, slightly reduced HA activity, mutant shows reduced fusion promotion activity | Avian orthoavulavirus 1 |
| R498Q | site-directed mutagenesis, mutation of a catalytic residue, highly reduced sialidase activity, slightly reduced HA activity, mutant shows reduced fusion promotion activity | Avian orthoavulavirus 1 |
| S418A | site-directed mutagenesis, mutation of a catalytic residue, unaltered sialidase activity, altered HA activity, mutant shows reduced fusion promotion activity | Avian orthoavulavirus 1 |
| Y262F | site-directed mutagenesis, mutation of a catalytic residue, retaining of nearly all sialidase activity, altered HA activity, mutant shows reduced fusion promotion activity | Avian orthoavulavirus 1 |
| Y262S | site-directed mutagenesis, mutation of a catalytic residue, loss of nearly all sialidase activity, highly reduced HA activity, mutant shows reduced fusion promotion activity | Avian orthoavulavirus 1 |
| Y317A | site-directed mutagenesis, mutation of a catalytic residue, loss of nearly all sialidase activity, highly reduced HA activity, mutant shows reduced fusion promotion activity | Avian orthoavulavirus 1 |
| Y317F | site-directed mutagenesis, mutation of a catalytic residue, reduced sialidase activity, altered HA activity, mutant shows reduced fusion promotion activity | Avian orthoavulavirus 1 |
| Y317S | site-directed mutagenesis, mutation of a catalytic residue, highly reduced sialidase activity, highly reduced HA activity, mutant shows reduced fusion promotion activity | Avian orthoavulavirus 1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | substrate inhibition | Avian orthoavulavirus 1 |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics and thermodynamics of wild-type and mutant enzymes | Avian orthoavulavirus 1 | |
| 0.5 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | pH 7.5, wild-type enzyme | Avian orthoavulavirus 1 | |
| 1.1 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | pH 7.5, mutant S418A | Avian orthoavulavirus 1 | |
| 4.9 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | pH 7.5, mutant Y262F | Avian orthoavulavirus 1 | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Avian orthoavulavirus 1 | 5886 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Avian orthoavulavirus 1 | Q9Q2W5 | Clone 30 strain | - |
| Avian orthoavulavirus 1 Kansas | Q9Q2W5 | Clone 30 strain | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| colominic acid + H2O = sialic acid + lactose | active site, Arg498, Glu258, Tyr317, and Tyr262 are catalytic residues, with the aromatic ring of the latter being important for function | Avian orthoavulavirus 1 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activities of diverse mutants, overview | Avian orthoavulavirus 1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Avian orthoavulavirus 1 | 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid | - |
? | |
| 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Avian orthoavulavirus 1 Kansas | 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid | - |
? | |
| additional information | multifunctional enzyme with sialidase, receptor-binding, and fusion promotion activities | Avian orthoavulavirus 1 | ? | - |
? | |
| additional information | multifunctional enzyme with sialidase, receptor-binding, and fusion promotion activities | Avian orthoavulavirus 1 Kansas | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| haemagglutinin-neuraminidase protein | - |
Avian orthoavulavirus 1 |
| HN | - |
Avian orthoavulavirus 1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Avian orthoavulavirus 1 |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.2 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | pH 7.5, mutant S418A | Avian orthoavulavirus 1 | |
| 24.7 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | pH 7.5, wild-type enzyme | Avian orthoavulavirus 1 | |
| 28.1 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | pH 7.5, mutant Y262F | Avian orthoavulavirus 1 | |
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