Cloned (Comment) | Organism |
---|---|
expression of recombinant enzyme in Trichoplusia ni insect cells via baculoirus infection, subcloning in Escherichia coli DH5alpha | influenza A virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | influenza A virus | |
0.021 | - |
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]pyridinium | pH 6.0, 37°C | influenza A virus | |
0.138 | - |
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3-methylpyridinium | pH 9.5, 37°C | influenza A virus | |
0.14 | - |
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3-methylpyridinium | pH 6.0, 37°C | influenza A virus | |
0.216 | - |
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]pyridinium | pH 9.5, 37°C | influenza A virus | |
0.261 | - |
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3,4-dimethylpyridinium | pH 9.5, 37°C | influenza A virus | |
0.385 | - |
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3,4-dimethylpyridinium | pH 6.0, 37°C | influenza A virus | |
0.45 | - |
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-4-methylpyridinium | pH 9.5, 37°C | influenza A virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
influenza A virus | - |
alpha-sialidase, influenza A/Tokyo/3/67 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from insect cells by density gradient centrifugation and gel filtration | influenza A virus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
colominic acid + H2O = sialic acid + lactose | reaction and kinetic mechanisms | influenza A virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | spontaneous hydrolysis of pyridinium salts of alpha-D-N-acetylneuraminic acid occurs via 2 different ways, effects on the enzymic reaction mechanism are investigated | influenza A virus | ? | - |
? | |
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3,4-dimethylpyridinium + H2O | - |
influenza A virus | sialic acid + 3,4-dimethylpyridine | - |
? | |
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3-methylpyridinium + H2O | - |
influenza A virus | sialic acid + 3-methylpyridine | - |
? | |
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-4-methylpyridinium + H2O | - |
influenza A virus | sialic acid + 4-methylpyridine | - |
? | |
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]pyridinium + H2O | - |
influenza A virus | sialic acid + pyridine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-sialidase | - |
influenza A virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | influenza A virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat at pH 9.5, 37°C, relative to the reported kcat at pH 6.0, 37°C, with substrate 4-nitrophenyl alpha-D-N-acetylneurminide | influenza A virus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | - |
influenza A virus |