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Literature summary for 3.2.1.18 extracted from

  • Chou, D.T.H.; Watson, J.N.; Scholte, A.A.; Borgford, T.J.; Bennet, A.J.
    Effect of neutral pyridine leaving groups on the mechanisms of influenza type A viral sialidase-catalyzed and spontaneous hydrolysis reactions of alpha-D-N-acetylneuraminides (2000), J. Am. Chem. Soc., 122, 8357-8364.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant enzyme in Trichoplusia ni insect cells via baculoirus infection, subcloning in Escherichia coli DH5alpha influenza A virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics influenza A virus
0.021
-
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]pyridinium pH 6.0, 37°C influenza A virus
0.138
-
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3-methylpyridinium pH 9.5, 37°C influenza A virus
0.14
-
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3-methylpyridinium pH 6.0, 37°C influenza A virus
0.216
-
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]pyridinium pH 9.5, 37°C influenza A virus
0.261
-
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3,4-dimethylpyridinium pH 9.5, 37°C influenza A virus
0.385
-
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3,4-dimethylpyridinium pH 6.0, 37°C influenza A virus
0.45
-
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-4-methylpyridinium pH 9.5, 37°C influenza A virus

Organism

Organism UniProt Comment Textmining
influenza A virus
-
alpha-sialidase, influenza A/Tokyo/3/67
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from insect cells by density gradient centrifugation and gel filtration influenza A virus

Reaction

Reaction Comment Organism Reaction ID
colominic acid + H2O = sialic acid + lactose reaction and kinetic mechanisms influenza A virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information spontaneous hydrolysis of pyridinium salts of alpha-D-N-acetylneuraminic acid occurs via 2 different ways, effects on the enzymic reaction mechanism are investigated influenza A virus ?
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3,4-dimethylpyridinium + H2O
-
influenza A virus sialic acid + 3,4-dimethylpyridine
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3-methylpyridinium + H2O
-
influenza A virus sialic acid + 3-methylpyridine
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-4-methylpyridinium + H2O
-
influenza A virus sialic acid + 4-methylpyridine
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]pyridinium + H2O
-
influenza A virus sialic acid + pyridine
-
?

Synonyms

Synonyms Comment Organism
alpha-sialidase
-
influenza A virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at influenza A virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information kcat at pH 9.5, 37°C, relative to the reported kcat at pH 6.0, 37°C, with substrate 4-nitrophenyl alpha-D-N-acetylneurminide influenza A virus

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5
-
influenza A virus