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Literature summary for 3.2.1.18 extracted from
- Purification and properties of cloned Salmonella typhimurium LT2 sialidase with virus-typical kinetic preference for sialyl alpha2-3 linkages (1991), J. Biochem., 110, 462-467.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overproduction in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Deoxy-2,3-didehydro-N-acetylneuraminic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.9 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 | 4-methylumbelliferone + alpha-D-N-acetylneuraminic acid | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 45 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | - |
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
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