Literature summary for 3.2.1.179 extracted from

Nakamichi, Y.; Maruyama, Y.; Mikami, B.; Hashimoto, W.; Murata, K.
Structural determinants in streptococcal unsaturated glucuronyl hydrolase for recognition of glycosaminoglycan sulfate groups (2011), J. Biol. Chem., 286, 6262-6271.

Search for more literature for 3.2.1.179

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants	Comment	Organism
G342S	kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is similar to the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to wild-type value	Bacillus sp. (in: Bacteria)
H339S	kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 1.2fold higher than the kcat/Km-value for the wild-type enzyme, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is 53% of the wild-type value	Bacillus sp. (in: Bacteria)
I344K	kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 6.1fold higher than the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is similar to the wild-type value	Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.381	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, wild-type enzyme	Bacillus sp. (in: Bacteria)
0.504	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, mutant enzyme G342S	Bacillus sp. (in: Bacteria)
0.566	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, mutant enzyme K370I	Bacillus sp. (in: Bacteria)
0.861	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, mutant enzyme H339S	Bacillus sp. (in: Bacteria)
2.2	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, mutant enzyme K370I	Bacillus sp. (in: Bacteria)
7	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, mutant enzyme G342S	Bacillus sp. (in: Bacteria)
7.19	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, mutant enzyme H339S	Bacillus sp. (in: Bacteria)
18.6	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, wild-type enzyme	Bacillus sp. (in: Bacteria)

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	Q9RC92	-	-
Bacillus sp. (in: Bacteria) GL1	Q9RC92	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O	-	Bacillus sp. (in: Bacteria)	4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine	-	?
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O	-	Bacillus sp. (in: Bacteria) GL1	4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine	-	?
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O	kcat/Km is 8% of the kcat/KM value for the unsulfated substrate beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	Bacillus sp. (in: Bacteria)	4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine-6-O-sulfate	-	?
beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O	kcat/Km is 8% of the kcat/KM value for the unsulfated substrate beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	Bacillus sp. (in: Bacteria) GL1	4-deoxy-L-threo-5-hexosulose-uronate + beta-D-N-acetylgalactosamine-6-O-sulfate	-	?

Synonyms

Synonyms	Comment	Organism
UGL	-	Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
14.1	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, wild-type enzyme	Bacillus sp. (in: Bacteria)
16.9	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, mutant enzyme H339S	Bacillus sp. (in: Bacteria)
18.2	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, mutant enzyme G342S	Bacillus sp. (in: Bacteria)
20.9	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, mutant enzyme G342S	Bacillus sp. (in: Bacteria)
23.8	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, mutant enzyme K370I	Bacillus sp. (in: Bacteria)
24.5	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, mutant enzyme H339S	Bacillus sp. (in: Bacteria)
39.8	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, mutant enzyme K370I	Bacillus sp. (in: Bacteria)
54.9	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, wild-type enzyme	Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Bacillus sp. (in: Bacteria)

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.95	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, wild-type enzyme	Bacillus sp. (in: Bacteria)
2.99	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, mutant enzyme G342S	Bacillus sp. (in: Bacteria)
3.41	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, mutant enzyme H339S	Bacillus sp. (in: Bacteria)
18.1	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S	pH 7.5, 30°C, mutant enzyme K370I	Bacillus sp. (in: Bacteria)
19.6	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, mutant enzyme H339S	Bacillus sp. (in: Bacteria)
36.1	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, mutant enzyme G342S	Bacillus sp. (in: Bacteria)
36.8	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, wild-type enzyme	Bacillus sp. (in: Bacteria)
42	-	beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc	pH 7.5, 30°C, mutant enzyme K370I	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)