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Literature summary for 3.2.1.178 extracted from

  • Hehemann, J.H.; Correc, G.; Barbeyron, T.; Helbert, W.; Czjzek, M.; Michel, G.
    Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota (2010), Nature, 464, 908-912.
    View publication on PubMed
Search for more literature for 3.2.1.178

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Zobellia galactanivorans

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of an inactivated mutant (E139S) of PorA in complex with a porphyran tetrasaccharide Zobellia galactanivorans
crystal structures of PorB in complex with a porphyran tetrasaccharide Zobellia galactanivorans

Protein Variants

Protein Variants Comment Organism
E139S inactive mutant enzyme Zobellia galactanivorans

Organism

Organism UniProt Comment Textmining
Zobellia galactanivorans D7GXF9
-
-
Zobellia galactanivorans D7GXG0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
porphyran + H2O the enzyme cleaves the (1->4) linkages between beta-D-galactopyranose and alpha-L-galactopyranose-6-sulfate, forming mostly the disaccharide alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactopyranoside, although some longer oligosaccharides of even number of residues are also observed. Since the enzyme is inactive on the non-sulfated agarose portion of the porphyran backbone, some agarose fragments are also included in the products Zobellia galactanivorans alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactopyranoside + ?
-
 ?
porphyran + H2O the enzyme cleaves the (1->4) linkages between beta-D-galactopyranose and alpha-L-galactopyranose-6-sulfate, forming mostly the disaccharide alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactose, although some longer oligosaccharides of even number of residues are also observed. Since the enzyme is inactive on the non-sulfated agarose portion of the porphyran backbone, some agarose fragments are also included in the products Zobellia galactanivorans alpha-L-galactopyranose-6-sulfate-(1->3)-beta-D-galactopyranoside + ?
-
 ?

Synonyms

Synonyms Comment Organism
PORA
-
 Zobellia galactanivorans
PORB
-
 Zobellia galactanivorans