Literature summary for 3.2.1.177 extracted from

Silipo, A.; Larsbrink, J.; Marchetti, R.; Lanzetta, R.; Brumer, H.; Molinaro, A.
NMR spectroscopic analysis reveals extensive binding interactions of complex xyloglucan oligosaccharides with the Cellvibrio japonicus glycoside hydrolase family 31 alpha-xylosidase (2012), Chemistry, 18, 13395-13404.

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Protein Variants

Protein Variants	Comment	Organism
D659A	site-directed mutagenesis, active site mutant, catalytically inactive variant	Cellvibrio japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O	Cellvibrio japonicus	-	D-xylose + beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4) D-Glc	-	?

Organism

Organism	UniProt	Comment	Textmining
Cellvibrio japonicus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O	-	Cellvibrio japonicus	D-xylose + beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4) D-Glc	-	?
additional information	the enzyme CjXyl31A demonstrates an exquisite regioselectivity for the terminal nonreducing-end alpha-(1->6)-xylose of xyloglucan oliogosaccharides	Cellvibrio japonicus	?	-	?
xyloglucan oligosaccharide + H2O	extensive interactions between the enzyme's active-site variants and xyloglucan hexa- and heptasaccharides. The enzyme recognizes the entire cello-tetraosyl backbone of the substrate and product in positive enzyme subsites and makes further significant interactions with internal pendant alpha-(1->6)-linked xylosyl units, role of the PA14 domain, stereochemistry of the hydrolysis, overview	Cellvibrio japonicus	?	-	?

Synonyms

Synonyms	Comment	Organism
alpha-xylosidase	-	Cellvibrio japonicus
alpha-xylosidase 31A	-	Cellvibrio japonicus
CjXyl31A	-	Cellvibrio japonicus

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the glycoside hydrolase family 31, GH31	Cellvibrio japonicus
additional information	extensive interactions between the enzyme's active-site variants and xyloglucan hexa- and heptasaccharides, role of the PA14 domain, structure-function analysis using a combination of NMR spectroscopic techniques, including saturation transfer difference and transfer NOE spectroscopy	Cellvibrio japonicus

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Release 2023.1 (January 2023)