Protein Variants | Comment | Organism |
---|---|---|
additional information | the carbohydrate-binding module has a dual effect on the activity, it diminishes the tendency of heat-induced desorption, but also has a pronounced negative effect on the maximal rate, which was 2fold larger in variants without carbohydrate-binding module throughout the investigated temperature range. Although the carbohydrate-binding module is beneficial for affinity it slows down the catalytic process | Trichoderma reesei |
additional information | the carbohydrate-binding module has a dual effect on the activity, it diminishes the tendency of heat-induced desorption, but also has a pronounced negative effect on the maximal rate, which was 2fold larger in variants without carbohydrate-binding module throughout the investigated temperature range. Although the carbohydrate-binding module is beneficial for affinity it slows down the catalytic process | Rasamsonia emersonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Avicel | Km value of the full-length enzyme increases from 2.8 g/l, pH 5.0, 10°C, to 19 g/l, pH 5.0, 50°C | Trichoderma reesei | |
additional information | - |
Avicel | Km value of the full-length enzyme increases from 2.8 g/l, pH 5.0, 10°C, to 29 g/l, pH 5.0, 50°C | Rasamsonia emersonii | |
additional information | - |
Avicel | Km value of the isolated catalytic domain increases from 4.9 g/l, pH 5.0, 10°C, to 76 g/l, pH 5.0, 50°C | Rasamsonia emersonii | |
additional information | - |
Avicel | Km value of the isolated catalytic domain increases from 7.1 g/l, pH 5.0, 10°C, to 122 g/l, pH 5.0, 50°C | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rasamsonia emersonii | Q8TFL9 | - |
- |
Trichoderma reesei | G0RVK1 | - |
- |
Trichoderma reesei QM6a | G0RVK1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | - |
Trichoderma reesei | cellobiose + ? | - |
? | |
avicel + H2O | - |
Rasamsonia emersonii | cellobiose + ? | - |
? | |
avicel + H2O | - |
Trichoderma reesei QM6a | cellobiose + ? | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the maximal rate increases strongly with temperature, whereas the affinity for the insoluble substrate decreases, and as a result, the effect of temperature depends strongly on the substrate load. Temperature has little or no effect on the hydrolytic rate in dilute substrate suspensions, whereas strong temperature activation is observed at saturating substrate loads | Trichoderma reesei |
additional information | - |
the maximal rate increases strongly with temperature, whereas the affinity for the insoluble substrate decreases, and as a result, the effect of temperature depends strongly on the substrate load. Temperature has little or no effect on the hydrolytic rate in dilute substrate suspensions, whereas strong temperature activation is observed at saturating substrate loads | Rasamsonia emersonii |