Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | overexpression of the protein disulfide isomerase Sc-PDI1 and the plasma membrane targeting soluble N-ethylmaleimide-sensitive factor attachment protein receptor Sc-SSO1, and disruption of the sorting receptor Sc-VPS10 and a Ca2D/Mn2D ATPase Sc-PMR1, improve respectively the extracellular Tr-Cel7A activities | Trichoderma reesei |
Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain CEN.PK102-3A | Trichoderma reesei |
gene cel7AF, recombinant expression of extracellular C-terminally FLAG-tagged enzyme with its native signal peptide in Saccharomyces cerevisiae strain CEN.PK102-3, the enzyme is secreted to the culture medium | Trichoderma reesei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | secretory pathway engineering, including protein folding, disulfide bond formation, and protein trafficking and sorting, enhances secretion of cellobiohydrolase I, with its native signal peptide, from Trichoderma reesei in Saccharomyces cerevisiae. Overexpression of the protein disulfide isomerase Sc-PDI1 and the plasma membrane targeting soluble N-ethylmaleimide-sensitive factor attachment protein receptor Sc-SSO1, and disruption of the sorting receptor Sc-VPS10 and a Ca2+/Mn2+ ATPase Sc-PMR1, improve respectively the extracellular Tr-Cel7A activities. The extracellular activities of the quadruple-modified strain using 4-nitrophenyl-beta-D-cellobioside and phosphoric acid swollen cellulose as the substrates, respectively, are 3.9fold and 1.3fold higher than that of the reference cel7AF strain, detailed overview | Trichoderma reesei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trichoderma reesei | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoric acid swollen cellulose + H2O | Trichoderma reesei | - |
cellobiose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | - |
- |
- |
Trichoderma reesei | G0RVK1 | gene cel7AF | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-cellobioside + H2O | - |
Trichoderma reesei | 4-methylumbelliferone + beta-D-cellobiose | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Trichoderma reesei | 4-nitrophenol + D-cellobiose | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Trichoderma reesei | cellobiose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cel7A | - |
Trichoderma reesei |
cellobiohydrolase I | - |
Trichoderma reesei |
Tr-Cel7A | - |
Trichoderma reesei |