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Literature summary for 3.2.1.176 extracted from

  • Xu, L.; Shen, Y.; Hou, J.; Peng, B.; Tang, H.; Bao, X.
    Secretory pathway engineering enhances secretion of cellobiohydrolase I from Trichoderma reesei in Saccharomyces cerevisiae (2014), J. Biosci. Bioeng., 117, 45-52.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information overexpression of the protein disulfide isomerase Sc-PDI1 and the plasma membrane targeting soluble N-ethylmaleimide-sensitive factor attachment protein receptor Sc-SSO1, and disruption of the sorting receptor Sc-VPS10 and a Ca2D/Mn2D ATPase Sc-PMR1, improve respectively the extracellular Tr-Cel7A activities Trichoderma reesei

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain CEN.PK102-3A Trichoderma reesei
gene cel7AF, recombinant expression of extracellular C-terminally FLAG-tagged enzyme with its native signal peptide in Saccharomyces cerevisiae strain CEN.PK102-3, the enzyme is secreted to the culture medium Trichoderma reesei

Protein Variants

Protein Variants Comment Organism
additional information secretory pathway engineering, including protein folding, disulfide bond formation, and protein trafficking and sorting, enhances secretion of cellobiohydrolase I, with its native signal peptide, from Trichoderma reesei in Saccharomyces cerevisiae. Overexpression of the protein disulfide isomerase Sc-PDI1 and the plasma membrane targeting soluble N-ethylmaleimide-sensitive factor attachment protein receptor Sc-SSO1, and disruption of the sorting receptor Sc-VPS10 and a Ca2+/Mn2+ ATPase Sc-PMR1, improve respectively the extracellular Tr-Cel7A activities. The extracellular activities of the quadruple-modified strain using 4-nitrophenyl-beta-D-cellobioside and phosphoric acid swollen cellulose as the substrates, respectively, are 3.9fold and 1.3fold higher than that of the reference cel7AF strain, detailed overview Trichoderma reesei

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Trichoderma reesei
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoric acid swollen cellulose + H2O Trichoderma reesei
-
cellobiose
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-
Trichoderma reesei G0RVK1 gene cel7AF
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-cellobioside + H2O
-
Trichoderma reesei 4-methylumbelliferone + beta-D-cellobiose
-
?
4-nitrophenyl beta-D-cellobioside + H2O
-
Trichoderma reesei 4-nitrophenol + D-cellobiose
-
?
phosphoric acid swollen cellulose + H2O
-
Trichoderma reesei cellobiose
-
?

Synonyms

Synonyms Comment Organism
Cel7A
-
Trichoderma reesei
cellobiohydrolase I
-
Trichoderma reesei
Tr-Cel7A
-
Trichoderma reesei