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Literature summary for 3.2.1.176 extracted from

  • Dror, T.W.; Morag, E.; Rolider, A.; Bayer, E.A.; Lamed, R.; Shoham, Y.
    Regulation of the cellulosomal CelS (cel48A) gene of Clostridium thermocellum is growth rate dependent (2003), J. Bacteriol., 185, 3042-3048.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.176

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the amount of CelS in the supernatant fluids of cellobiose-grown cultures is lower than that of cellulose-grown cultures Acetivibrio thermocellus
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Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellulose + H2O
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 Acetivibrio thermocellus ?
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 ?

Synonyms

Synonyms Comment Organism
Cel48A
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 Acetivibrio thermocellus
celS
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 Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
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 assay at Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Acetivibrio thermocellus

Expression

Organism Comment Expression
Acetivibrio thermocellus celS appears to be regulated at the transcriptional level, and its expression is modulated by growth rate both under conditions of cellobiose and nitrogen limitation. The amount of celS mRNA transcripts per cell is about 180 for cells grown under carbon limitation at growth rates of 0.04 to 0.21 per h and 80 and 30 transcripts per cell for batch cultures at growth rates of 0.23 and 0.35 per hour, respectively. Under nitrogen limitation, the corresponding levels were 110, 40, and 30 transcripts/cell for growth rates of 0.07, 0.11, and 0.14 per hour, respectively. Two major transcriptional start sites are detected at positions 140 and 145 bp, upstream of the translational start site of the celS gene. The relative activity of the two promoters remains constant under the conditions studied and is in agreement with the results of the RNase protection assay, in which the observed transcriptional activity is inversely proportional to the growth rate down
Acetivibrio thermocellus celS appears to be regulated at the transcriptional level, and its expression is modulated by growth rate both under conditions of cellobiose and nitrogen limitation. The amount of celS mRNA transcripts per cell is about 180 for cells grown under carbon limitation at growth rates of 0.04 to 0.21 per h and 80 and 30 transcripts per cell for batch cultures at growth rates of 0.23 and 0.35 per hour, respectively. Under nitrogen limitation, the corresponding levels were 110, 40, and 30 transcripts/cell for growth rates of 0.07, 0.11, and 0.14 per hour, respectively. Two major transcriptional start sites are detected at positions 140 and 145 bp, upstream of the translational start site of the celS gene. The relative activity of the two promoters remains constant under the conditions studied and is in agreement with the results of the RNase protection assay, in which the observed transcriptional activity is inversely proportional to the growth rate up