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Literature summary for 3.2.1.175 extracted from

  • Kato-Noguchi, H.; Tanaka, Y.
    Effect of ABA-beta-D-glucopyranosyl ester and activity of ABA-beta-D-glucosidase in Arabidopsis thaliana (2008), J. Plant Physiol., 165, 788-790.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
beta-D-glucopyranosyl abscisate pH 6.0, 35°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucopyranosyl abscisate + H2O Arabidopsis thaliana librated free abscisate may induce growth inhibition in Arabidopsis hypocotyls. Exogenously applied beta-D-glucopyranosyl abscisate may be absorbed by roots and hydrolyzed by beta-D-glucosidase, with the subsequent release of abscisate, which would potentially inhibit the growth of Arabidopsis hypocotyls beta-D-glucopyranose + abscisate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SE50 L. ecotype Columbia
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.017
-
pH 6.0, 35°C, activity in control seedlings Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucopyranosyl abscisate + H2O
-
Arabidopsis thaliana beta-D-glucopyranose + abscisate
-
?
beta-D-glucopyranosyl abscisate + H2O librated free abscisate may induce growth inhibition in Arabidopsis hypocotyls. Exogenously applied beta-D-glucopyranosyl abscisate may be absorbed by roots and hydrolyzed by beta-D-glucosidase, with the subsequent release of abscisate, which would potentially inhibit the growth of Arabidopsis hypocotyls Arabidopsis thaliana beta-D-glucopyranose + abscisate
-
?

Synonyms

Synonyms Comment Organism
ABA-beta-D-glucosidase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
enzyme in crude extract Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana at the concentration of 100 mM beta-D-glucopyranosyl abscisate, the activity is 1.7fold greater than in controls, which suggests that beta-D-glucopyranosyl abscisate might cause enzyme induction up