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Literature summary for 3.2.1.173 extracted from

  • Mutter, M.; Beldman, G.; Pitson, S.M.; Schols, H.A.; Voragen, A.G.
    Rhamnogalacturonan alpha-D-galactopyranosyluronohydrolase. An enzyme that specifically removes the terminal nonreducing galacturonosyl residue in rhamnogalacturonan regions of pectin (1998), Plant Physiol., 117, 153-163.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.173

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha 40°C, pH 5.0 Aspergillus aculeatus
0.085
-
 alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha 40°C, pH 5.0 Aspergillus aculeatus
0.21
-
 alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha 40°C, pH 5.0 Aspergillus aculeatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66000, SDS-PAGE Aspergillus aculeatus

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus aculeatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha + H2O
-
 Aspergillus aculeatus beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha
-
 ?
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha + H2O
-
 Aspergillus aculeatus beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha
-
 ?
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]4-(1->2)-alpha-L-Rha + H2O
-
 Aspergillus aculeatus beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]4-(1->2)-alpha-L-Rha
-
 ?
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha + H2O
-
 Aspergillus aculeatus beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha
-
 ?
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]6-(1->2)-alpha-L-Rha + H2O
-
 Aspergillus aculeatus beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]6-(1->2)-alpha-L-Rha
-
 ?
additional information no activity with: CM-cellulose, crystalline cellulose, xylan from oat spelts, soluble starch, potato arabino-beta-(1,4)-galactan, larchwood arabino-beta-(1,3)/(1,6)-galactan (stractan), linear arabinan, polygalacturonic acid with or without 1 mM CaCl2 added, 4-nitrophenyl-alpha-L-Araf, 4-nitrophenyl-alpha-D-Galp, 4-nitrophenyl-beta-D-Galp, 4-nitrophenyl-alpha-D-Xylp, 4-nitrophenyl-beta-D-Xylp, 4-nitrophenyl-alpha-D-Manp, 4-nitrophenylb-D-Manp, 4-nitrophenyl-alpha-L-Fucp, 4-nitrophenyl-beta-D-Fucp, 4-nitrophenyl-alpha-D-Glcp, 4-nitrophenyl-beta-D-Glcp, 4-nitrophenyl-alpha-L-Rhap, 4-nitrophenyl-beta-D-GlcpA, and 4-nitrophenyl-beta-D-GalpA Aspergillus aculeatus ?
-
 ?
rhamnogalacturonan I oligosaccharides with alpha-D-GalA at the non-reducing end + H2O active toward all rhamnogalacturonan types of substrate, except for those with a 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end Aspergillus aculeatus beta-D-galacturonic acid + shortened rhamnogalacturonan I oligosaccharides with beta-D-galacturonic acid at the reducing end and at the nonreducing en
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Aspergillus aculeatus

Synonyms

Synonyms Comment Organism
RG-galacturonohydrolase
-
 Aspergillus aculeatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 NaOAc buffer, pH 5.0 Aspergillus aculeatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable for at least 3 h Aspergillus aculeatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
 alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha 40°C, pH 5.0 Aspergillus aculeatus
1.8
-
 alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha 40°C, pH 5.0 Aspergillus aculeatus
2.4
-
 alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha 40°C, pH 5.0 Aspergillus aculeatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 McIlvaine buffer Aspergillus aculeatus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 4.5 pH 3.5: about 75% of maximal activity, pH 4.5: about 75% of maximal activity Aspergillus aculeatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 7 most stable for 1 h at 40°C Aspergillus aculeatus
3 6 most stable for 24 h at 40°C Aspergillus aculeatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus aculeatus in isoelectric focusing a major band at pI 5.12 and minor bands at 5.0, 5.07, and 5.2 are found. Preparative isoelectric focusing shows maximal activity of RG-galacturonohydrolase in the collected fractions with a pH of 4.9 and 5.0
-
 5.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11
-
 alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha 40°C, pH 5.0 Aspergillus aculeatus
20
-
 alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha 40°C, pH 5.0 Aspergillus aculeatus
21
-
 alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha 40°C, pH 5.0 Aspergillus aculeatus