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Literature summary for 3.2.1.172 extracted from

  • Itoh, T.; Ochiai, A.; Mikami, B.; Hashimoto, W.; Murata, K.
    A novel glycoside hydrolase family 105: the structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a disaccharide in comparison with family 88 enzyme complexed with the disaccharide (2006), J. Mol. Biol., 360, 573-585.
    View publication on PubMed
Search for more literature for 3.2.1.172

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, crystal structure of YteR complexed with unsaturated chondroitin disaccharide (substrate of bacterial unsaturated glucuronyl hydrolase). The substrate is sterically hindered with the active pocket of YteR. The protruding loop of YteR prevents the UGL substrate from being bound effectively Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 4.0 Bacillus subtilis
0.719
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 6.0 Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 gel filtration Bacillus subtilis
43000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O Bacillus subtilis the enzyme is involved in the degradation of the rhamnogalacturonan I main chain in Bacillus subtilis 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O Bacillus subtilis 168 the enzyme is involved in the degradation of the rhamnogalacturonan I main chain in Bacillus subtilis 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O31521
-
-
Bacillus subtilis O34559
-
-
Bacillus subtilis 168 O31521
-
-
Bacillus subtilis 168 O34559
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O
-
 Bacillus subtilis 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O the enzyme is involved in the degradation of the rhamnogalacturonan I main chain in Bacillus subtilis Bacillus subtilis 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O
-
 Bacillus subtilis 168 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O the enzyme is involved in the degradation of the rhamnogalacturonan I main chain in Bacillus subtilis Bacillus subtilis 168 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O
-
 Bacillus subtilis 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose
-
 ?
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O
-
 Bacillus subtilis 168 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose
-
 ?
additional information no activity with: DELTAGlcA-GalNAc, DELTAGlcA-GlcNAc, and DELTAGalA-(GalA)n Bacillus subtilis ?
-
 ?
additional information no activity with: DELTAGlcA-GalNAc, DELTAGlcA-GlcNAc, and DELTAGalA-(GalA)n Bacillus subtilis 168 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Bacillus subtilis
monomer 1 * 39000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
YesR
-
 Bacillus subtilis
YteR
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable below Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 4.0 Bacillus subtilis
13.9
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 6.0 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Bacillus subtilis
6
-
-
 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
4.8 7.2 pH 4.8: about 40% of maximal activity, pH 7.2: about 55% of maximal activity Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 4.0 Bacillus subtilis
19.3
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 6.0 Bacillus subtilis