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Literature summary for 3.2.1.172 extracted from

  • Itoh, T.; Ochiai, A.; Mikami, B.; Hashimoto, W.; Murata, K.
    Structure of unsaturated rhamnogalacturonyl hydrolase complexed with substrate (2006), Biochem. Biophys. Res. Commun., 347, 1021-1029.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, structure of D143N in complex with unsaturated rhamnogalacturonan disaccharide substrate 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose is determined at 1.9 A resolution by X-ray crystallography Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D143N inactive mutant enzyme Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34559
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Bacillus subtilis 168 O34559
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O postulation of the enzyme reaction mechanism: YteR triggers the hydration of vinyl ether group in 4-deoxy-beta-L-threo-hex-4-enopyranuronose (DELTAGalA), but not of glycoside bond, by using Asp143 as a general acid and base catalyst. Asp143 donates proton to the double bond of 4-deoxy-beta-L-threo-hex-4-enopyranuronose as an acid catalyst and also deprotonates a water molecule as a base catalyst. Deprotonated water molecule attacks the C5 atom of 4-deoxy-beta-L-threo-hex-4-enopyranuronose Bacillus subtilis 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose
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2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O postulation of the enzyme reaction mechanism: YteR triggers the hydration of vinyl ether group in 4-deoxy-beta-L-threo-hex-4-enopyranuronose (DELTAGalA), but not of glycoside bond, by using Asp143 as a general acid and base catalyst. Asp143 donates proton to the double bond of 4-deoxy-beta-L-threo-hex-4-enopyranuronose as an acid catalyst and also deprotonates a water molecule as a base catalyst. Deprotonated water molecule attacks the C5 atom of 4-deoxy-beta-L-threo-hex-4-enopyranuronose Bacillus subtilis 168 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose
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?

Synonyms

Synonyms Comment Organism
YteR
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Bacillus subtilis