Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, structure of D143N in complex with unsaturated rhamnogalacturonan disaccharide substrate 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose is determined at 1.9 A resolution by X-ray crystallography | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D143N | inactive mutant enzyme | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O34559 | - |
- |
Bacillus subtilis 168 | O34559 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O | postulation of the enzyme reaction mechanism: YteR triggers the hydration of vinyl ether group in 4-deoxy-beta-L-threo-hex-4-enopyranuronose (DELTAGalA), but not of glycoside bond, by using Asp143 as a general acid and base catalyst. Asp143 donates proton to the double bond of 4-deoxy-beta-L-threo-hex-4-enopyranuronose as an acid catalyst and also deprotonates a water molecule as a base catalyst. Deprotonated water molecule attacks the C5 atom of 4-deoxy-beta-L-threo-hex-4-enopyranuronose | Bacillus subtilis | 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose | - |
? | |
2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O | postulation of the enzyme reaction mechanism: YteR triggers the hydration of vinyl ether group in 4-deoxy-beta-L-threo-hex-4-enopyranuronose (DELTAGalA), but not of glycoside bond, by using Asp143 as a general acid and base catalyst. Asp143 donates proton to the double bond of 4-deoxy-beta-L-threo-hex-4-enopyranuronose as an acid catalyst and also deprotonates a water molecule as a base catalyst. Deprotonated water molecule attacks the C5 atom of 4-deoxy-beta-L-threo-hex-4-enopyranuronose | Bacillus subtilis 168 | 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
YteR | - |
Bacillus subtilis |