Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Pichia pastoris X-33 | Irpex lacteus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme contains a secretion signal sequence of 22 amino acids | Irpex lacteus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44600 | - |
x * 44600, about, sequence calculation, x * 56000, recombinant enzyme, SDS-PAGE | Irpex lacteus |
56000 | - |
x * 44600, about, sequence calculation, x * 56000, recombinant enzyme, SDS-PAGE | Irpex lacteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Irpex lacteus | B6E8Y7 | - |
- |
Irpex lacteus KY2902 | B6E8Y7 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.8 | - |
purified recombinant enzyme, substrate acetylated rhamnogalacturonan, pH 5.0, 40°C | Irpex lacteus |
75 | - |
purified recombinant enzyme, substrate non-acetylated rhamnogalacturonan, pH 5.0, 40°C | Irpex lacteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylated rhamnogalacturonan + H2O | - |
Irpex lacteus | ? | - |
? | |
acetylated rhamnogalacturonan + H2O | - |
Irpex lacteus KY2902 | ? | - |
? | |
non-acetylated rhamnogalacturonan + H2O | - |
Irpex lacteus | ? | - |
? | |
non-acetylated rhamnogalacturonan + H2O | - |
Irpex lacteus KY2902 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44600, about, sequence calculation, x * 56000, recombinant enzyme, SDS-PAGE | Irpex lacteus |
Synonyms | Comment | Organism |
---|---|---|
RG-hydrolase | - |
Irpex lacteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | recombinant enzyme | Irpex lacteus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | activity range, overview | Irpex lacteus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant enzyme, stable up to | Irpex lacteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | recombinant enzyme | Irpex lacteus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | activity range, overview | Irpex lacteus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6 | purified recombinant enzyme, 90 min 50% to 60% of the activity remaining | Irpex lacteus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Irpex lacteus | sequence calculation | - |
6.2 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 28, GH28 | Irpex lacteus |