Literature summary for 3.2.1.17 extracted from

Kyomuhendo, P.; Myrnes, B.; Brandsdal, B.O.; Smalas, A.O.; Nilsen, I.W.; Helland, R.
Thermodynamics and structure of a salmon cold active goose-type lysozyme (2010), Comp. Biochem. Physiol. B, 156, 254-263.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, hanging drop vapour diffusion method, 0.001 ml of 8 mg/ml protein in 150 mM NaCl and 50 mM HEPES, pH 7.25, is mixed with 0.001 ml of reservoir solutions containing 43-45% ammonium sulfate, 0.01 M cobalt chloride, and 0.1 M MES, pH 6.25-6.5, 22°C, 3 weeks, X-ray diffraction structure determination and analysis at 1.75 A resolution	Salmo salar

Organism

Organism	UniProt	Comment	Textmining
Salmo salar	A6PZ97	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
unfolding and potential refolding analysis due to cold adaptation and heat treatment, differential scanning calorimetry. Recombinant SalG has a melting temperature of 36.8°C under thermal denaturation conditions and regains activity after returning to permissive (low) temperature. Refolding is dramatically reduced in solutions with high SalG concentrations, coupled with significant protein precipitation. Rapid and irreversible inactivation takes place during heating at high enzyme concentration	Salmo salar

Renatured (Comment)

Organism

unfolding and potential refolding analysis due to cold adaptation and heat treatment, differential scanning calorimetry. Recombinant SalG has a melting temperature of 36.8°C under thermal denaturation conditions and regains activity after returning to permissive (low) temperature. Refolding is dramatically reduced in solutions with high SalG concentrations, coupled with significant protein precipitation. Rapid and irreversible inactivation takes place during heating at high enzyme concentration

Salmo salar

Subunits

Subunits	Comment	Organism
More	structure homology modeling, overview	Salmo salar

Synonyms

Synonyms	Comment	Organism
Goose-type lysozyme	-	Salmo salar
SalG	-	Salmo salar

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	unfolding and potential refolding analysis due to cold adaptation and heat treatment, differential scanning calorimetry. Recombinant SalG has a melting temperature of 36.8°C under thermal denaturation conditions and regains activity after returning to permissive (low) temperature. Rapid and irreversible inactivation takes place during heating at high enzyme concentration	Salmo salar