Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.168 extracted from

  • Mazzaferro, L.S.; Pinuel, L.; Erra-Balsells, R.; Giudicessi, S.L.; Breccia, J.D.
    Transglycosylation specificity of Acremonium sp. alpha-rhamnosyl-beta-glucosidase and its application to the synthesis of the new fluorogenic substrate 4-methylumbelliferyl-rutinosid (2012), Carbohydr. Res., 347, 69-75.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
DMSO enzyme hydrolytic activity is not modified by addition of 2% v/v DMSO and is significantly diminished at DMSO concentration of 10% v/v. After 18 h incubation the yield of product is nervertheless increased by 2fold; hydrolytic activity tested with 1.8 mM hesperidin as substrate. Activity is not modified by addition of 2% (v/v) DMSO, but is significantly diminished when the concentration is increased up to 10% (v/v). Hydrolysis yield is increased upon addition of DMSO, with hesperetin final concentrations in the range of 0.47-0.95 mM after 18 h reaction Acremonium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hesperidin + H2O Acremonium sp.
-
hesperetin + rutinose
-
?

Organism

Organism UniProt Comment Textmining
Acremonium sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
hesperidin + H2O = hesperetin + rutinose diglycosidase activity, catalyzing the transglycosylation and hydrolysis reactions Acremonium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hesperidin + H2O
-
Acremonium sp. hesperetin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside i.e. hesperetin + rutinose ?
hesperidin + H2O
-
Acremonium sp. hesperetin + rutinose
-
?
additional information the enzyme shows a high alpha-rhamnosyl-beta-glucosidase transglycosylation activity with hesperidin and 4-methylumbelliferone resulting in formation of 4-methylumbelliferyl-rutinoside nd hesperetin, overview Acremonium sp. ?
-
?

Synonyms

Synonyms Comment Organism
alpha-rhamnosyl-beta-glucosidase
-
Acremonium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Acremonium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Acremonium sp.