Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DMSO | enzyme hydrolytic activity is not modified by addition of 2% v/v DMSO and is significantly diminished at DMSO concentration of 10% v/v. After 18 h incubation the yield of product is nervertheless increased by 2fold; hydrolytic activity tested with 1.8 mM hesperidin as substrate. Activity is not modified by addition of 2% (v/v) DMSO, but is significantly diminished when the concentration is increased up to 10% (v/v). Hydrolysis yield is increased upon addition of DMSO, with hesperetin final concentrations in the range of 0.47-0.95 mM after 18 h reaction | Acremonium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hesperidin + H2O | Acremonium sp. | - |
hesperetin + rutinose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium sp. | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
hesperidin + H2O = hesperetin + rutinose | diglycosidase activity, catalyzing the transglycosylation and hydrolysis reactions | Acremonium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hesperidin + H2O | - |
Acremonium sp. | hesperetin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside | i.e. hesperetin + rutinose | ? | |
hesperidin + H2O | - |
Acremonium sp. | hesperetin + rutinose | - |
? | |
additional information | the enzyme shows a high alpha-rhamnosyl-beta-glucosidase transglycosylation activity with hesperidin and 4-methylumbelliferone resulting in formation of 4-methylumbelliferyl-rutinoside nd hesperetin, overview | Acremonium sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-rhamnosyl-beta-glucosidase | - |
Acremonium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Acremonium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Acremonium sp. |