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Literature summary for 3.2.1.168 extracted from

  • Mazzaferro, L.; Pinuel, L.; Minig, M.; Breccia, J.D.
    Extracellular monoenzyme deglycosylation system of 7-O-linked flavonoid beta-rutinosides and its disaccharide transglycosylation activity from Stilbella fimetaria (2010), Arch. Microbiol., 192, 383-393.
    View publication on PubMed
Search for more literature for 3.2.1.168

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.77
-
 hesperidin pH 5.0, 60°C Acremonium sp.
8.73
-
 hesperidin methylchalcone pH 5.0, 60°C Acremonium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cell bound
-
 Acremonium sp.
-
-
extracellular
-
 Acremonium sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ reduces the activity in the range of 10-50% Acremonium sp.
Ca2+ reduces the activity in the range of 10-50% Acremonium sp.
Cu2+ deleterious for enzyme activity Acremonium sp.
EDTA reduces the activity in the range of 10-50% Acremonium sp.
Fe2+ reduces the activity in the range of 10-50% Acremonium sp.
Hg2+ deleterious for enzyme activity Acremonium sp.
Mg2+ reduces the activity in the range of 10-50% Acremonium sp.
additional information no influence of manganese on the enzyme activity Acremonium sp.
Ni2+ reduces the activity in the range of 10-50% Acremonium sp.
Zn2+ reduces the activity in the range of 10-50% Acremonium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42220
-
 mass spectrometry Acremonium sp.
46000
-
 SDS-PAGE Acremonium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hesperidin + H2O Acremonium sp. pH 5.0, 60°C hesperetin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol increasing ethanol concentration 0-20% shows to diminish hesperidin hydrolysis, but total activity, hydrolysis plus transglycosylation activity increases Acremonium sp.

Organism

Organism UniProt Comment Textmining
Acremonium sp.
-
 SES201, isolated from South Patagonia, Argentina
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Acremonium sp.

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, fractionation with ammonium sulfate and two chromatographic steps: butyl-agarose and QAE-Sephadex Acremonium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diosmin + H2O a 7-O-beta-rutinoside, pH 5.0, 60°C, 3.3% relative activity compared to hesperidin as substrate Acremonium sp. didymin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?
eriocitrin + H2O a 7-O-beta-rutinoside, pH 5.0, 60°C, 89.7% relative activity compared to hesperidin as substrate Acremonium sp. eriodictyol + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?
hesperidin + H2O pH 5.0, 60°C Acremonium sp. hesperetin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?
hesperidin + H2O i.e. hesperitin 7-(6-O-alpha-rhamnopyranosyl)-beta-D-glucopyranoside, pH 5.0, 60°C, substrates and products of the enzymatic reaction are analyzed by UV-MALDI-TOF/TOF mass spectrometry Acremonium sp. hesperetin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside i.e. hesperetin + rutinose, substrates and products of the enzymatic reaction are analyzed by UV-Maldi-TOF/TOF mass spectrometry ?
hesperidin methylchalcone + H2O a 7-O-beta-rutinoside, pH 5.0, 60°C, 58.5% relative activity compared to hesperidin as substrate Acremonium sp. hesperetin methylchalcone + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?
additional information in addition, when incubated with 5% (v/v) ethanol as acceptor and hesperidin and hesperidin methylchalcone as sugar donors another glycosylated product is found as a result of a transglycosylation reaction: ethyl rutinoside, indicating that alpha-rhamnosyl-beta-glucosidase is able to transfer disaccharide units to OH-acceptors in an aqueous medium Acremonium sp. ?
-
 ?
additional information no dectectable enzyme activity using the 7-O-beta-neohesperidosides: naringin and neohesperidin as substrates, or the 3-O-beta-rutinoside rutin Acremonium sp. ?
-
 ?
narirutin + H2O a 7-O-beta-rutinoside, pH 5.0, 60°C, 61% relative activity compared to hesperidin as substrate Acremonium sp. naringenin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 also active at 5°C, Tm of 74.6°C and a calculated activation energy Ea of 183.3 kJ/mol Acremonium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: ca. 20% relative activity, 80°C: ca. 50% relative activity Acremonium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
74.6
-
 Tm of 74.6°C and a calculated activation energy Ea of 183.3 kJ/mol Acremonium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
32.4
-
 hesperidin pH 5.0, 60°C Acremonium sp.
68.2
-
 hesperidin methylchalcone pH 5.0, 60°C Acremonium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Acremonium sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pH 4: ca. 70% relative activity, pH 8: ca. 60% relative activity Acremonium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Acremonium sp. isoelectric focusing
-
 5.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.8
-
 hesperidin methylchalcone pH 5.0, 60°C Acremonium sp.
18.3
-
 hesperidin pH 5.0, 60°C Acremonium sp.