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Literature summary for 3.2.1.167 extracted from
- Purification and characterization of baicalin-beta-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge (2005), Proc. Biochem., 40, 1911-1915.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 50 mM, 1.2 fold activation | Scutellaria viscidula |  |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | synthesis of baicalein (i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one), a main active ingredient of Scutellaria sp. used in traditional Chinese medicine. It is difficult to obtain baicalein directly from skullaps because of its low content | Scutellaria viscidula |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 10 mM: 14% inhibition | Scutellaria viscidula | |
| Fe3+ | 10 mM: 50% inhibition | Scutellaria viscidula | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Ca2+ and Zn2+ have no significant effect on enzyme activity | Scutellaria viscidula |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58400 | - |
x * 58400, SDS-PAGE | Scutellaria viscidula |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Scutellaria viscidula | - |
Bge | - |
| Scutellaria viscidula Bge | - |
Bge | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Scutellaria viscidula |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Scutellaria viscidula | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.36 | - |
- |
Scutellaria viscidula |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O | i.e. baicalin. No activity with p-nitrophenyl-beta-D-glucoside. Activity with p-nitrophenyl-beta-D-glucuronide is about 4% of the activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid (baicalin), activity with p-phenolphthalein-beta-D-glucuronide is 9.5% of the activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid (baicalin) | Scutellaria viscidula | 5,6,7-trihydroxyflavone + D-glucuronate | i.e. baicalein | ? | |
| 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O | i.e. baicalin. No activity with p-nitrophenyl-beta-D-glucoside. Activity with p-nitrophenyl-beta-D-glucuronide is about 4% of the activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid (baicalin), activity with p-phenolphthalein-beta-D-glucuronide is 9.5% of the activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid (baicalin) | Scutellaria viscidula Bge | 5,6,7-trihydroxyflavone + D-glucuronate | i.e. baicalein | ? | |
| 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O | i.e. wogonin 7-O-beta-D-glucuronide | Scutellaria viscidula | 5,7-dihydroxy-8-methoxyflavone + D-glucuronate | - |
? | |
| 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O | i.e. wogonin 7-O-beta-D-glucuronide | Scutellaria viscidula Bge | 5,7-dihydroxy-8-methoxyflavone + D-glucuronate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58400, SDS-PAGE | Scutellaria viscidula |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| baicalin-beta-D-glucuronidase | - |
Scutellaria viscidula |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Scutellaria viscidula |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | 40°C: about 60% of maximal activity, 70°C: about 50% of maximal activity | Scutellaria viscidula |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
30 min, pH 5.0, stable below | Scutellaria viscidula |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Scutellaria viscidula |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | pH 3.0: about 50% of maximal activity, pH 7.0: about 65% of maximal activity | Scutellaria viscidula |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
30 min, about 50% loss of activity | Scutellaria viscidula |
| 5 | 5.5 | 30 min, stable | Scutellaria viscidula |
| 6 | - |
30 min, about 35% loss of activity | Scutellaria viscidula |
| 7 | - |
30 min, about 40% of maximal activity | Scutellaria viscidula |
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