Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.166 extracted from

  • He, Y.Q.; Sutcliffe, E.L.; Bunting, K.L.; Li, J.; Goodall, K.J.; Poon, I.K.; Hulett, M.D.; Freeman, C.; Zafar, A.; McInnes, R.L.; Taya, T.; Parish, C.R.; Rao, S.
    The endoglycosidase heparanase enters the nucleus of T lymphocytes and modulates H3 methylation at actively transcribed genes via the interplay with key chromatin modifying enzymes (2012), Transcription, 3, 130-145.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.166

Protein Variants

Protein Variants Comment Organism
additional information enzyme silencing by different siRNAs, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information heparanase occupies regulatory regions of active genes in T cells Homo sapiens
-
-
nucleus nuclear heparanase preferentially associates with euchromatin in active and resting Jurkat T cells. Heparanase is recruited to both the promoter and transcribed regions of a distinct cohort of transcriptionally active genes. Endogenous heparanase forms a complex with RNAP II, histone H3 (a key nucleosome component) and the H3K9ac activation mark, in resting and activated T cells Homo sapiens 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8000
-
 1 * 53000 + 1 * 8000, active heterodimeric form, SDS-PAGE Homo sapiens
53000
-
 1 * 53000 + 1 * 8000, active heterodimeric form, SDS-PAGE Homo sapiens
65000
-
 1 * 65000, inactive pro-form, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens heparanase is an endo-beta-glucuronidase that cleaves heparan sulfate and facilitates the passage of migrating cells through extra­cellular matrices, particularly basement membranes, as well as releasing heparan sulfate-bound growth factors from the extracellular matrices, whereby the released growth factors also aid wound heal­ing and angiogenesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
B-16 cell
-
 Homo sapiens
-
blood platelet
-
 Homo sapiens
-
JURKAT cell
-
 Homo sapiens
-
MCF-7 cell
-
 Homo sapiens
-
MDA-MB-231 cell
-
 Homo sapiens
-
MOLT-4 cell
-
 Homo sapiens
-
T-lymphocyte heparanase occupies regulatory regions of active genes in T cells Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information heparanase is an endo-beta-glucuronidase that cleaves heparan sulfate and facilitates the passage of migrating cells through extra­cellular matrices, particularly basement membranes, as well as releasing heparan sulfate-bound growth factors from the extracellular matrices, whereby the released growth factors also aid wound heal­ing and angiogenesis Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 53000 + 1 * 8000, active heterodimeric form, SDS-PAGE Homo sapiens
monomer 1 * 65000, inactive pro-form, SDS-PAGE Homo sapiens

Expression

Organism Comment Expression
Homo sapiens phorbol 12-myristate 13-acetate/calcium iono­mycin stimulation results in a substantial increase in nuclear heparanase in the 53 kDa active form up

General Information

General Information Comment Organism
malfunction in siRNA treated T-cells, the repressing H3K9ac activation mark is reduced, with transcriptional repression of the CD69, IFNalpha and IL-2 genes and a concomitant decrease in H3K4me1 levels, accumulation of H3K4m2, not H3K4m3 Homo sapiens
additional information endogenous heparanase forms a complex with RNAP II, histone H3 (a key nucleosome component) and the H3K9ac activation mark, in resting and activated T cells and with euchromatin Homo sapiens
physiological function nuclear heparanase controls transcription of a distinct cohort of T-cell inducible genes. Since heparanase associates with active chromatin marks and RNAP II, the activated enzyme plays a role in gene transcription. The endoglycosidase heparanase enters the nucleus of T lymphocytes and modulates H3 methylation at actively transcribed genes via the interplay with key chromatin modifying enzymes. Chromatin-bound heparanase is a prerequisite for the transcription of a subset of inducible immune response genes in activated T-cells. The actions of heparanase seem to influence gene transcription by associating with the demethylase LSD1, preventing recruitment of the methylase MLL and thereby modifying histone H3 methylation patterns. Heparanase belongs to an emerging class of proteins that play an important role in regulating transcription in addition to their well-recognized extra-nuclear functions Homo sapiens