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Literature summary for 3.2.1.165 extracted from

  • Chao, C.F.; Chen, Y.Y.; Cheng, C.Y.; Li, Y.K.
    Catalytic function of a newly purified exo-beta-D-glucosaminidase from the entomopathogenic fungus Paecilomyces lilacinus (2013), Carbohydr. Polym., 93, 615-621.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
-
Purpureocillium lilacinum

Organism

Organism UniProt Comment Textmining
Purpureocillium lilacinum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by cation-exchange chromatography from chitosan-grown cells Purpureocillium lilacinum

Reaction

Reaction Comment Organism Reaction ID
hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini two-step mechanism and transglycosylation of the retaining glycoside hydrolase forming a glycosyl-enzyme intermediate Purpureocillium lilacinum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
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Purpureocillium lilacinum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitobiose + H2O (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product Purpureocillium lilacinum glucosamine + glucosamine
-
?
chitohexaose + H2O (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product Purpureocillium lilacinum chitopentaose + glucosamine
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?
chitopentaose + H2O (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product Purpureocillium lilacinum chitotetraose + glucosamine
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?
chitotetraose + H2O (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product Purpureocillium lilacinum chitotriose + glucosamine
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?
chitotriose + H2O (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product Purpureocillium lilacinum chitobiose + D-glucosamine
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?
additional information the enzyme cleaves the beta-1->4-glycosidic bonds between GlcN-GlcN and GlcN-GlcNAc but not between GlcNAc-GlcN or GlcNAc-GlcNAc, cleavage of GlcN-GlcNAc, a partially acetylated chitooligomer mixture, overview. The purified enzyme displays high activity toward chitosan and (GlcN)6. No significant activity with N-carboxymethyl chitosan, or chitin, also synthetic p-nitrophenyl-beta-D-glucosaminide is not hydrolyzed. Chitosan with higher deacetylation degrees is hydrolyzed more efficiently than chitosan with lower deacetylation values. Substrate specifiicty analysis using massspectrometry. Paecilomyces exo-beta-D-GlcNase can accelerate the hydrolysis of p-nitrophenyl-beta-D-N-acetylglucosaminide, p-nitrophenyl-beta-D-N,N'-diacetylchitobioside, and p-nitrophenyl-beta-D-N-acetylgalactosaminide to release the corresponding sugar Purpureocillium lilacinum ?
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?

Synonyms

Synonyms Comment Organism
exo-beta--D-GlcNase
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Purpureocillium lilacinum
exo-beta-D-glucosaminidase
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Purpureocillium lilacinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Purpureocillium lilacinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Purpureocillium lilacinum

Expression

Organism Comment Expression
Purpureocillium lilacinum the enzyme is induced by chitosan up