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Literature summary for 3.2.1.165 extracted from

  • van Bueren, A.L.; Ghinet, M.G.; Gregg, K.; Fleury, A.; Brzezinski, R.; Boraston, A.B.
    The structural basis of substrate recognition in an exo-beta-D-glucosaminidase involved in chitosan hydrolysis (2009), J. Mol. Biol., 385, 131-139.
    View publication on PubMed
Search for more literature for 3.2.1.165

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces lividans Amycolatopsis orientalis

Crystallization (Commentary)

Crystallization (Comment) Organism
E541A mutant in complex with a natural β-1,4-D-glucosamine tetrasaccharide substrate and both E541A and D469A mutants in complex with a pNP-beta-D-glucosaminide synthetic substrate Amycolatopsis orientalis

Protein Variants

Protein Variants Comment Organism
D469A catalytic acid/base Amycolatopsis orientalis
E541A catalytic nucleophile Amycolatopsis orientalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
 predicted molecular mass from atomic model, confirmed by SDS-PAGE of recombinant scxA gene product lacking carbohydrate-binding module Amycolatopsis orientalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-D-glucosamine tetrasaccharide Amycolatopsis orientalis tetrasaccharide of chitosan efficiently depolymerizes chitosan to its constituent p-glucosamine monosaccharide in cooperation with endo-chitosanase D-glucosamine + chitotriose
-
 ?
additional information Amycolatopsis orientalis cleaving nonreducing beta-D-glucosamine residues of oligosaccharides, highly specific due to pocket for N2 group of glucosaminidase discriminating it from glucose ?
-
 ?

Organism

Organism UniProt Comment Textmining
Amycolatopsis orientalis Q56F26
-
-

Purification (Commentary)

Purification (Comment) Organism
from culture supernatant Amycolatopsis orientalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-D-glucosamine tetrasaccharide tetrasaccharide of chitosan efficiently depolymerizes chitosan to its constituent p-glucosamine monosaccharide in cooperation with endo-chitosanase Amycolatopsis orientalis D-glucosamine + chitotriose
-
 ?
additional information cleaving nonreducing beta-D-glucosamine residues of oligosaccharides, highly specific due to pocket for N2 group of glucosaminidase discriminating it from glucose Amycolatopsis orientalis ?
-
 ?
p-nitrophenyl-beta-D-glucosamine + H2O
-
 Amycolatopsis orientalis D-glucosamine + p-nitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
CsxA
-
 Amycolatopsis orientalis
exo-beta-D-glucosaminidase
-
 Amycolatopsis orientalis
exo-chitosanase
-
 Amycolatopsis orientalis