Literature summary for 3.2.1.165 extracted from

Fukamizo, T.; Fleury, A.; Cote, N.; Mitsutomi, M.; Brzezinski, R.
Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism (2006), Glycobiology, 16, 1064-1072.

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Application

Application	Comment	Organism
synthesis	efficient tool for industrial production of glucosamine monosaccharide	Amycolatopsis orientalis

Protein Variants

Protein Variants	Comment	Organism
D469A	inactive mutant enzyme	Amycolatopsis orientalis
D469E	inactive mutant enzyme	Amycolatopsis orientalis
E541D	inactive mutant enzyme	Amycolatopsis orientalis
E541Q	inactive mutant enzyme	Amycolatopsis orientalis
S468N/D469E	inactive mutant enzyme	Amycolatopsis orientalis

Organism

Organism	UniProt	Comment	Textmining
Amycolatopsis orientalis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chitotetraose + H2O	at first, the enzyme predominantly produces D-glucosamine and chitotriose, which is further degraded into D-glucosamine and chitobiose. The transglycosylation product, chitopentaose, is also produced, together with a lesser amount of chitohexaose	Amycolatopsis orientalis	D-glucosamine	-	?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + H2O	(GlcN)3-GlcNAc (mono-N-acetylated chitotetraose, the reducing end residue is N-acetylated) is at first degraded into (GlcN)2-GlcNAc, and then into GlcN-GlcNAc. After a longer incubation period, the substrate is finally hydrolyzed into monosaccharides, GlcN and GlcNAc	Amycolatopsis orientalis	GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + D-glucosamine	-	?

Synonyms

Synonyms	Comment	Organism
exo-beta-D-glucosaminidase	-	Amycolatopsis orientalis

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Release 2023.1 (January 2023)