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Literature summary for 3.2.1.165 extracted from
- One-step preparation of alkyl beta-D-glucosaminide by the transglycosylation of chitosan and alcohol using purified exo-beta-D-glucosaminidase (1999), Biotechnol. Lett., 21, 451-456.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
chitotetraose | - |
Talaromyces funiculosus |  |
| 0.96 | - |
chitotriose | - |
Talaromyces funiculosus | |
| 1.4 | - |
chitobiose | - |
Talaromyces funiculosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Talaromyces funiculosus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 108000 | - |
x * 108000, SDS-PAGE | Talaromyces funiculosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces funiculosus | - |
- |
- |
| Talaromyces funiculosus KY616 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Talaromyces funiculosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Talaromyces funiculosus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41.2 | - |
- |
Talaromyces funiculosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitobiose + H2O | - |
Talaromyces funiculosus | D-glucosamine | - |
? | |
| chitobiose + H2O | - |
Talaromyces funiculosus KY616 | D-glucosamine | - |
? | |
| chitosan + H2O | only D-glucosamine is produced after prolonged hydrolysis | Talaromyces funiculosus | D-glucosamine + ? | - |
? | |
| chitosan + H2O | only D-glucosamine is produced after prolonged hydrolysis | Talaromyces funiculosus KY616 | D-glucosamine + ? | - |
? | |
| chitotetraose + H2O | - |
Talaromyces funiculosus | D-glucosamine | - |
? | |
| chitotetraose + H2O | - |
Talaromyces funiculosus KY616 | D-glucosamine | - |
? | |
| chitotriitol + H2O | - |
Talaromyces funiculosus | D-glucosamine | - |
? | |
| chitotriitol + H2O | - |
Talaromyces funiculosus KY616 | D-glucosamine | - |
? | |
| chitotriose + H2O | - |
Talaromyces funiculosus | chitobiose + D-glucosamine | - |
? | |
| additional information | the enzyme also catalyzes transglycosylation reactions | Talaromyces funiculosus | ? | - |
? | |
| additional information | the enzyme also catalyzes transglycosylation reactions | Talaromyces funiculosus KY616 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 108000, SDS-PAGE | Talaromyces funiculosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-beta-D-GlcNase | - |
Talaromyces funiculosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | substrate: chitosan | Talaromyces funiculosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
substrate: chitosan | Talaromyces funiculosus |
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