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Literature summary for 3.2.1.165 extracted from

  • Ike, M.; Isami, K.; Tanabe, Y.; Nogawa, M.; Ogasawara, W.; Okada, H.; Morikawa, Y.
    Cloning and heterologous expression of the exo-beta-D-glucosaminidase-encoding gene (gls93) from a filamentous fungus, Trichoderma reesei PC-3-7 (2006), Appl. Microbiol. Biotechnol., 72, 687-695.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Trichoderma reesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 x * 100000, the recombinant Gls93 has the two molecular forms, ca. 105000 Da and 100000 Da, whose difference is caused by N-glycosylation, SDS-PAGE Trichoderma reesei
105000
-
 x * 105000, the recombinant Gls93 has the two molecular forms, ca. 105000 Da and 100000 Da, whose difference is caused by N-glycosylation, SDS-PAGE Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei Q4R1C4 precursor
-
Trichoderma reesei PC-3-7 Q4R1C4 precursor
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the recombinant Gls93 has the two molecular forms, ca. 105000 Da and 100000 Da, whose difference is caused by N-glycosylation Trichoderma reesei

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Trichoderma reesei

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate of cells grown on N-acetyl-D-glucosamine Trichoderma reesei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitopentaose + H2O cleaved to chitotetraose and glucosamine in the initial stage of the reaction Trichoderma reesei ?
-
 ?
chitopentaose + H2O cleaved to chitotetraose and glucosamine in the initial stage of the reaction Trichoderma reesei PC-3-7 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, the recombinant Gls93 has the two molecular forms, ca. 105000 Da and 100000 Da, whose difference is caused by N-glycosylation, SDS-PAGE Trichoderma reesei
? x * 105000, the recombinant Gls93 has the two molecular forms, ca. 105000 Da and 100000 Da, whose difference is caused by N-glycosylation, SDS-PAGE Trichoderma reesei

Synonyms

Synonyms Comment Organism
exo-beta-D-glucosaminidase
-
 Trichoderma reesei
Gls93
-
 Trichoderma reesei