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Literature summary for 3.2.1.165 extracted from

  • Uchida, Y.; Takeda, H.; Ohkuma, A.; Seki, K.
    Purification and properties of exo-beta-D-glucosaminidase from Penicillium sp. and its applications (1997), Adv. Chitin Sci., 2, 244-249.
No PubMed abstract available
Search for more literature for 3.2.1.165

Application

Application Comment Organism
synthesis enzymatic formation of chitooligosaccharides by transglycosylation Penicillium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 1 * 110000, SDS-PAGE Penicillium sp.
125000
-
 gel filtration Penicillium sp.

Organism

Organism UniProt Comment Textmining
Penicillium sp.
-
-
-
Penicillium sp. AF9-P-112
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Penicillium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39.66
-
-
 Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitohexaitol + H2O 89% of the activity with chitopentaitol Penicillium sp. D-glucosamine + ?
-
 ?
chitohexaitol + H2O 89% of the activity with chitopentaitol Penicillium sp. AF9-P-112 D-glucosamine + ?
-
 ?
chitopentaitol + H2O
-
 Penicillium sp. D-glucosamine + ?
-
 ?
chitopentaitol + H2O
-
 Penicillium sp. AF9-P-112 D-glucosamine + ?
-
 ?
chitosan + H2O soluble chitosan, powdered chitosan and colloidal chitosan Penicillium sp. D-glucosamine + ?
-
 ?
chitosan + H2O soluble chitosan, powdered chitosan and colloidal chitosan Penicillium sp. AF9-P-112 D-glucosamine + ?
-
 ?
chitotetraitol + H2O 97% of the activity with chitopentaitol Penicillium sp. D-glucosamine + ?
-
 ?
chitotetraitol + H2O 97% of the activity with chitopentaitol Penicillium sp. AF9-P-112 D-glucosamine + ?
-
 ?
chitotriitol + H2O 85% of the activity with chitopentaitol Penicillium sp. D-glucosamine + ?
-
 ?
glycol chitosan + H2O
-
 Penicillium sp. D-glucosamine + ?
-
 ?
additional information the enzyme catalyzes transglycosylation reaction on chitobiose and chitotriose Penicillium sp. ?
-
 ?
additional information the enzyme catalyzes transglycosylation reaction on chitobiose and chitotriose Penicillium sp. AF9-P-112 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 110000, SDS-PAGE Penicillium sp.

Synonyms

Synonyms Comment Organism
exo-beta-D-glucosaminidase
-
 Penicillium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Penicillium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable below Penicillium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Penicillium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 stable Penicillium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium sp.
-
-
 7.4