Literature summary for 3.2.1.161 extracted from

Chuankhayan, P.; Rimlumduan, T.; Tantanuch, W.; Mothong, N.; Kongsaeree, P.T.; Metheenukul, P.; Svasti, J.; Jensen, O.N.; Cairns, J.R.
Functional and structural differences between isoflavonoid beta-glycosidases from Dalbergia sp (2007), Arch. Biochem. Biophys., 468, 205-16.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris	Dalbergia nigrescens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.8	-	dalpatein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside	pH 5.5, 30°C, recombinant Dnbglu2	Dalbergia nigrescens
4	-	dalnigrein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside	pH 5.5, 30°C, recombinant Dnbglu2	Dalbergia nigrescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60510	-	monomer, calculated from protein sequence	Dalbergia nigrescens
240000	-	native protein, gel filtration chromatography	Dalbergia nigrescens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dalnigrein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside + H2O	Dalbergia nigrescens	-	dalnigrein + beta-D-apiofuranosyl-(1->6)-D-glucose + H+	-	?
dalpatein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside + H2O	Dalbergia nigrescens	-	dalpatein + beta-D-apiofuranosyl-(1->6)-D-glucose + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Dalbergia nigrescens	A3RF67	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dalnigrein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside + H2O	-	Dalbergia nigrescens	dalnigrein + beta-D-apiofuranosyl-(1->6)-D-glucose + H+	-	?
dalpatein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside + H2O	-	Dalbergia nigrescens	dalpatein + beta-D-apiofuranosyl-(1->6)-D-glucose + H+	-	?

Subunits

Subunits	Comment	Organism
homotetramer	-	Dalbergia nigrescens

Synonyms

Synonyms	Comment	Organism
Dnbglu2	-	Dalbergia nigrescens
isoflavonoid 7-O-apiosylglucosidase	-	Dalbergia nigrescens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
20	-	dalpatein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside	pH 5.5, 30°C, recombinant Dnbglu2	Dalbergia nigrescens
229	-	dalnigrein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside	pH 5.5, 30°C, recombinant Dnbglu2	Dalbergia nigrescens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5.32	-	dalpatein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside	pH 5.5, 30°C, recombinant Dnbglu2	Dalbergia nigrescens
58.6	-	dalnigrein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside	pH 5.5, 30°C, recombinant Dnbglu2	Dalbergia nigrescens

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Release 2023.1 (January 2023)