Cloned (Comment) | Organism |
---|---|
gene encoding iota-carrageenase flanked by a C-terminal hexahistidine tag and a N-terminal PelB signal peptide for targeting the gene product into Escherichia coli periplasm. The recombinant plasmid, referred to as pETIAf, is used to transform Escherichia coli BL21(DE3) strain harbouring pLysS plasmid | Alteromonas fortis |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method using polyethylene glycol (MW = 6000) as a precipitant. Crystals belong to space group P2(1), with unit-cell parameters a = 56.75 A, b = 91.04 A, c = 125.01 A, beta = 93.4°. The unit cell contains two molecules in the asymmetric unit related by a non-crystallographic twofold axis. Crystals diffracted to 2.0 A resolution on a synchrotron beamline | Alteromonas fortis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alteromonas fortis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Alteromonas fortis |