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Literature summary for 3.2.1.155 extracted from
- Specific xyloglucanases as a new class of polysaccharide-degrading enzymes (2004), Biochim. Biophys. Acta, 1674, 268-281.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM-value for xyloglucan is 0.3 mg/ml, KM-value for beta-glucan is 8 mg/ml, KM-value for carboxymethylcellulose is above 51 mg/mL | Trichoderma reesei | |
| additional information | - |
additional information | KM-value for xyloglucan is 0.31 mg/ml, KM-value for beta-glucan is 18 mg/ml, KM-value for carboxymethylcellulose is above 50 mg/mL | Chrysosporium lucknowense |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, SDS-PAGE | Aspergillus japonicus |
| 78000 | - |
x * 78000, SDS-PAGE | Chrysosporium lucknowense |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xyloglucan + H2O | Aspergillus japonicus | - |
xyloglucan oligosaccharides | - |
? |  |
| xyloglucan + H2O | Chrysosporium lucknowense | - |
xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | Trichoderma reesei | - |
xyloglucan oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus japonicus | - |
- |
- |
| Chrysosporium lucknowense | - |
- |
- |
| Trichoderma reesei | Q7Z9M8 | - |
- |
| Trichoderma reesei | Q7Z9M8 | Cel74A; gene cel74, several enzyme forms | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 2 putative N-glycosylation sites in Cel74A | Trichoderma reesei |
| glycoprotein | enzyme contains two putative N-glycosylation sites. There may be different forms of the enzyme resulting from O-glycosylation | Trichoderma reesei |
| additional information | Cel74A contains a signal peptide | Trichoderma reesei |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ion exchange chromatography, to homogeneity | Aspergillus japonicus |
| further purification of the commercial preparation, by ion exchange chromatography, to homogeneity | Chrysosporium lucknowense |
| further purification of the commercial preparation, by ion exchange chromatography, to homogeneity | Trichoderma reesei |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Hydrolysis of (1->4)-D-glucosidic linkages in xyloglucans so as to successively remove oligosaccharides from the chain end. | endo-type reaction mode, overview | Aspergillus japonicus | |
| Hydrolysis of (1->4)-D-glucosidic linkages in xyloglucans so as to successively remove oligosaccharides from the chain end. | exo-type reaction mode, overview | Chrysosporium lucknowense | |
| Hydrolysis of (1->4)-D-glucosidic linkages in xyloglucans so as to successively remove oligosaccharides from the chain end. | exo-type reaction mode, overview | Trichoderma reesei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Chrysosporium lucknowense | - |
| commercial preparation | - |
Trichoderma reesei | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl xyloglucan derivatives + H2O | artificial substrate | Trichoderma reesei | oligosaccharides | - |
? | |
| beta-glucan + H2O | 8% of the activity with xyloglucan | Chrysosporium lucknowense | beta-glucan oligosaccharides | - |
? | |
| beta-glucan + H2O | 8% of the activity with xyloglucan | Trichoderma reesei | beta-glucan oligosaccharides | - |
? | |
| carboxymethyl cellulose + H2O | 5% of the activity with xyloglucan | Chrysosporium lucknowense | carboxymethyl cellulose oligosaccharides | - |
? | |
| carboxymethyl cellulose + H2O | 6% of the activity with xyloglucan | Trichoderma reesei | carboxymethyl cellulose oligosaccharides | - |
? | |
| carboxymethylcellulose + H2O | - |
Trichoderma reesei | carboxymethyl cellulose oligosaccharides | - |
? | |
| additional information | substrate specificity, carboxymethylcellulose and barley beta-glucan are poor or no substrates | Aspergillus japonicus | ? | - |
? | |
| additional information | substrate specificity, carboxymethylcellulose and barley beta-glucan are poor or no substrates | Chrysosporium lucknowense | ? | - |
? | |
| additional information | substrate specificity, carboxymethylcellulose and barley beta-glucan are poor or no substrates | Trichoderma reesei | ? | - |
? | |
| tamarind xyloglucan + H2O | highly specific for, exo-type reaction | Trichoderma reesei | oligosaccharides | with end products XXXG, XXLG/XLXG, and XLLG | ? | |
| tamarind xyloglucan + H2O | highly specific for, endo-type reaction | Aspergillus japonicus | oligosacchrides | with end products XXXG, XXLG/XLXG, and XLLG | ? | |
| tamarind xyloglucan + H2O | highly specific for, exo-type reaction | Chrysosporium lucknowense | oligosacchrides | with end products XXXG, XXLG/XLXG, and XLLG | ? | |
| xyloglucan + H2O | - |
Aspergillus japonicus | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | - |
Chrysosporium lucknowense | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | - |
Trichoderma reesei | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | - |
Trichoderma reesei | xyloglucan oligosaccharides | XXXG, XXLG and XLLG oligosaccharides | ? | |
| xyloglucan + H2O | the enzyme is a true endo-glucanase when it acts on linear substrates without bulky substituents in the polymer backbone. When the enzyme acts on polymeric substrate with bulky side chains, its mode of action is switched to exo-like | Chrysosporium lucknowense | xyloglucan oligosaccharides | XXXG, XXLG and XLLG oligosaccharides | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78000, SDS-PAGE | Chrysosporium lucknowense |
| ? | x * 32000, SDS-PAGE | Aspergillus japonicus |
| ? | x * 75000-105000, several enzyme forms, SDS-PAGE | Trichoderma reesei |
| ? | x * 75000-105000, isoelectrofocusing, there may be different forms of the enzyme resulting from O-glycosylation, SDS-PAGE | Trichoderma reesei |
| More | peptide fingerprinting | Trichoderma reesei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel74A | - |
Trichoderma reesei |
| XG | - |
Aspergillus japonicus |
| XG | - |
Chrysosporium lucknowense |
| XG | - |
Trichoderma reesei |
| xyloglucanase | - |
Aspergillus japonicus |
| xyloglucanase | - |
Chrysosporium lucknowense |
| xyloglucanase | - |
Trichoderma reesei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Trichoderma reesei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 5.0, stable for at least 24 h | Aspergillus japonicus |
| 40 | - |
pH 5.0, stable for at least 24 h | Chrysosporium lucknowense |
| 40 | - |
pH 5.0, stable for at least 24 h | Trichoderma reesei |
| 50 | - |
half-life: 24 h | Chrysosporium lucknowense |
| 50 | - |
half-life: 24 h | Trichoderma reesei |
| 50 | - |
pH 5.0, half-life below 24 h | Aspergillus japonicus |
| 50 | - |
pH 5.0, half-life below 24 h | Chrysosporium lucknowense |
| 50 | - |
pH 5.0, half-life below 24 h | Trichoderma reesei |
| 60 | - |
half-life: 15 min | Trichoderma reesei |
| 60 | - |
half-life: 18 min | Chrysosporium lucknowense |
| 60 | - |
pH 5.0, half-life 15 min | Trichoderma reesei |
| 60 | - |
pH 5.0, half-life 18 min | Chrysosporium lucknowense |
| 60 | - |
pH 5.0, half-life 28 h | Aspergillus japonicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
beta-Glucan | - |
Trichoderma reesei | |
| 30 | - |
beta-Glucan | - |
Chrysosporium lucknowense | |
| 46 | - |
carboxymethylcellulose | - |
Trichoderma reesei | |
| 55 | - |
xyloglucan | - |
Trichoderma reesei | |
| 99 | - |
xyloglucan | - |
Chrysosporium lucknowense | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Aspergillus japonicus |
| 5 | 6 | - |
Chrysosporium lucknowense |
| 5 | 6 | - |
Trichoderma reesei |
| 5.3 | - |
- |
Trichoderma reesei |
| 6 | - |
- |
Chrysosporium lucknowense |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.3 | 6.9 | more than 50% of maximal activity in the pH-range 4.5-7.2 | Trichoderma reesei |
| 4.5 | 7.2 | more than 50% of maximal activity in the pH-range 4.5-7.2 | Chrysosporium lucknowense |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
40°C, stable for at least 24 h | Chrysosporium lucknowense |
| 5 | - |
40°C, stable for at least 24 h | Trichoderma reesei |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus japonicus | isoelectric focusing | - |
2.8 |
| Chrysosporium lucknowense | isoelectric focusing | - |
3.8 |
| Chrysosporium lucknowense | isoelectrofocusing | - |
3.8 |
| Trichoderma reesei | isoelectric focusing, several enzyme forms | 4.3 | 4.1 |
| Trichoderma reesei | isoelectrofocusing, there may be different forms of the enzyme resulting from O-glycosylation | 4.3 | 4.1 |
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Release 2023.1 (January 2023)
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