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Literature summary for 3.2.1.154 extracted from

  • Le Roy, K.; Lammens, W.; Van Laere, A.; Van den Ende, W.
    Influencing the binding configuration of sucrose in the active sites of chicory fructan 1-exohydrolase and sugar beet fructan 6-exohydrolase (2008), New Phytol., 178, 572-580.
    View publication on PubMed
Search for more literature for 3.2.1.154

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Pichia pastoris, pPICZalphaA vector Beta vulgaris

Protein Variants

Protein Variants Comment Organism
F233D introduction of acidic amino acid in vicinity of acid-base catalyst creating a beta-fructofuranosidase type of enzyme with dual activity against sucrose and levan, generally lower activities against levan and 6-kestose but conserving preference for these substrates over sucrose Beta vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Beta vulgaris hydrolytic cleavage of terminal fructosyl residues off inulin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Beta vulgaris Q70XE6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Beta vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-kestose + H2O
-
 Beta vulgaris ?
-
 ?
levan + H2O
-
 Beta vulgaris ?
-
 ?
levansucrase + H2O Bacillus subtilis Beta vulgaris ?
-
 ?
additional information hydrolytic cleavage of terminal fructosyl residues off inulin Beta vulgaris ?
-
 ?
sucrose + H2O
-
 Beta vulgaris D-fructose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
6-FEH
-
 Beta vulgaris
fructan 6-exohydrolase
-
 Beta vulgaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 sucrose 5 mM substrate: about zero acitivity in wild-type, mutant about 0.1 mol fructose/mol enzyme, at 1000 mM strongly raised activity: 4 mol fructose/mol enzyme Beta vulgaris
additional information
-
 levan 5 mM substrate: wild-type 6-FEH about 2.2 mol fructose/mol enzyme, F233D mutant about 0.4 mol fructose/mol enzyme Beta vulgaris
additional information
-
 6-kestose 5 mM substrate: wild-type enzyme about 1.8 mol fructose/mol enzyme, mutant about 0.3 mol fructose/mol enzyme Beta vulgaris