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Literature summary for 3.2.1.154 extracted from
- Purification and characterisation of an extracellular fructan beta-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish (2002), Syst. Appl. Microbiol., 25, 13-20.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 4 mM, 32% inhibition | Lactiplantibacillus pentosus |  |
| Cu2+ | 4 mM, 39% inhibition | Lactiplantibacillus pentosus | |
| EDTA | 4 mM, 60% inhibition | Lactiplantibacillus pentosus | |
| Fe2+ | 4 mM, 94% inhibition | Lactiplantibacillus pentosus | |
| Mg2+ | 4 mM, 42% inhibition | Lactiplantibacillus pentosus | |
| PCMB | 4 mM, complete inhibition | Lactiplantibacillus pentosus | |
| Zn2+ | 4 mM, 33% inhibition | Lactiplantibacillus pentosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Lactiplantibacillus pentosus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 125000 | - |
1 * 125000, SDS-PAGE | Lactiplantibacillus pentosus |
| 126000 | - |
gel filtration | Lactiplantibacillus pentosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus pentosus | - |
B235 | - |
| Lactiplantibacillus pentosus B235 | - |
B235 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactiplantibacillus pentosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 260.9 | - |
- |
Lactiplantibacillus pentosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,1,1-kestose + H2O | 45% of the activity with levan | Lactiplantibacillus pentosus | ? | - |
? | |
| 1,1-kestose + H2O | 39% of the activity with levan | Lactiplantibacillus pentosus | ? | - |
? | |
| 1-kestose + H2O | 12% of the activity with levan | Lactiplantibacillus pentosus | ? | - |
? | |
| garlic fructan + H2O | 60% of the activity with levan | Lactiplantibacillus pentosus | ? | - |
? | |
| inulin + H2O | 9% of the activity with levan | Lactiplantibacillus pentosus | ? | - |
? | |
| levan + H2O | best substrate | Lactiplantibacillus pentosus | fructose + ? | - |
? | |
| sucrose + H2O | 3% of the activity with levan | Lactiplantibacillus pentosus | D-fructose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 125000, SDS-PAGE | Lactiplantibacillus pentosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Lactiplantibacillus pentosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | 10°C: 60% of maximal activity, 40°C: about 50% of maximal activity | Lactiplantibacillus pentosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Lactiplantibacillus pentosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7 | pH 3.5: about 55% of maximal activity, pH 7.0: about 60% of maximal activity | Lactiplantibacillus pentosus |
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